Bis(5'-adenosyl)-triphosphatase ENPP4

UniProt data

UniProt name: ENPP4_HUMAN (Q9Y6X5)

Gene name: ENPP4; Synonyms=KIAA0879, NPP4

Organism: Homo sapiens (Human).

Function: Hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. Ap3A and Ap4A are diadenosine polyphosphates thought to induce proliferation of vascular smooth muscle cells. Acts as a procoagulant, mediating platelet aggregation at the site of nascent thrombus via release of ADP from Ap3A and activation of ADP receptors. {ECO:0000269|PubMed:22995898, ECO:0000269|PubMed:24338010}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:22995898}; Single-pass type I membrane protein {ECO:0000269|PubMed:22995898}.

Transmembrane domains:

  1. 408-428, 21 AA, Helical. {ECO:0000255}.

Sequence: 453 AA

10 20 30 40 50 60 MKLLVILLFS GLITGFRSDS SSSLPPKLLL VSFDGFRADY LKNYEFPHLQ NFIKEGVLVE 70 80 90 100 110 120 HVKNVFITKT FPNHYSIVTG LYEESHGIVA NSMYDAVTKK HFSDSNDKDP FWWNEAVPIW 130 140 150 160 170 180 VTNQLQENRS SAAAMWPGTD VPIHDTISSY FMNYNSSVSF EERLNNITMW LNNSNPPVTF 190 200 210 220 230 240 ATLYWEEPDA SGHKYGPEDK ENMSRVLKKI DDLIGDLVQR LKMLGLWENL NVIITSDHGM 250 260 270 280 290 300 TQCSQDRLIN LDSCIDHSYY TLIDLSPVAA ILPKINRTEV YNKLKNCSPH MNVYLKEDIP 310 320 330 340 350 360 NRFYYQHNDR IQPIILVADE GWTIVLNESS QKLGDHGYDN SLPSMHPFLA AHGPAFHKGY 370 380 390 400 410 420 KHSTINIVDI YPMMCHILGL KPHPNNGTFG HTKCLLVDQW CINLPEAIAI VIGSLLVLTM 430 440 450 LTCLIIIMQN RLSVPRPFSR LQLQEDDDDP LIG

CATM prediction

The sequence of ENPP4_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       410       420       43
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 AEAIAIVIGSLLVLTMLTCLIIIMQNR 7.2 -51.0° 3.3 48.1 -4.7
Prolines* PEAIAIVIGSLLVLTMLTCLIIIMQNR          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 410, ILE, HA A, 409, ALA, O 3.02Å
B, 413, GLY, HA1 A, 410, ILE, O 3.11Å
A, 410, ILE, HA B, 409, ALA, O 3.02Å
A, 413, GLY, HA1 B, 410, ILE, O 3.11Å