Heparan sulfate glucosamine 3-O-sulfotransferase 4

UniProt data

UniProt name: HS3S4_HUMAN (Q9Y661)

Gene name: HS3ST4; Synonyms=3OST4

Organism: Homo sapiens (Human).

Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N- unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Unlike 3-OST-1, does not convert non- anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity). {ECO:0000250}.

Subcellular location: Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 35-59, 25 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 456 AA

10 20 30 40 50 60 MARWPAPPPP PPPPPPLAAP PPPGASAKGP PARKLLFMCT LSLSVTYLCY SLLGGSGSLQ 70 80 90 100 110 120 FPLALQESPG AAAEPPPSPP PPSLLPTPVR LGAPSQPPAP PPLDNASHGE PPEPPEQPAA 130 140 150 160 170 180 PGTDGWGLPS GGGGAQDAWL RTPLAPSEMI TAQSALPERE AQESSTTDED LAGRRAANGS 190 200 210 220 230 240 SERGGAVSTP DYGEKKLPQA LIIGVKKGGT RALLEAIRVH PDVRAVGVEP HFFDRNYEKG 250 260 270 280 290 300 LEWYRNVMPK TLDGQITMEK TPSYFVTNEA PKRIHSMAKD IKLIVVVRNP VTRAISDYTQ 310 320 330 340 350 360 TLSKKPEIPT FEVLAFKNRT LGLIDASWSA IRIGIYALHL ENWLQYFPLS QILFVSGERL 370 380 390 400 410 420 IVDPAGEMAK VQDFLGLKRV VTEKHFYFNK TKGFPCLKKP EDSSAPRCLG KSKGRTHPRI 430 440 450 DPDVIHRLRK FYKPFNLMFY QMTGQDFQWE QEEGDK

CATM prediction

The sequence of HS3S4_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
          40        50        60 
   +    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 ARKLLFMCTLSLSVTYLCYSLLGGSGSLQFA 6.9 -48.8° 2.8 32.0 -1.5
Prolines* ARKLLFMCTLSLSVTYLCYSLLGGSGSLQFP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 47, TYR, HH A, 46, THR, OG1 2.34Å
B, 51, SER, HA A, 50, TYR, O 3.26Å
B, 54, GLY, HA1 A, 51, SER, O 2.96Å
B, 55, GLY, HA2 A, 58, SER, OG 3.12Å
A, 47, TYR, HH B, 46, THR, OG1 2.34Å
A, 51, SER, HA B, 50, TYR, O 3.26Å
A, 54, GLY, HA1 B, 51, SER, O 2.96Å
A, 55, GLY, HA2 B, 58, SER, OG 2.70Å