N-acetylated-alpha-linked acidic dipeptidase 2

UniProt data

UniProt name: NALD2_HUMAN (Q9Y3Q0)

Gene name: NAALAD2

Organism: Homo sapiens (Human).

Function: Has N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Also exhibits a dipeptidyl-peptidase IV type activity. Inactivate the peptide neurotransmitter N- acetylaspartylglutamate. {ECO:0000269|PubMed:10085079}.

Subcellular location: Cell membrane {ECO:0000250|UniProtKB:Q04609}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q04609}.

Transmembrane domains:

  1. 8-31, 24 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 740 AA

10 20 30 40 50 60 MAESRGRLYL WMCLAAALAS FLMGFMVGWF IKPLKETTTS VRYHQSIRWK LVSEMKAENI 70 80 90 100 110 120 KSFLRSFTKL PHLAGTEQNF LLAKKIQTQW KKFGLDSAKL VHYDVLLSYP NETNANYISI 130 140 150 160 170 180 VDEHETEIFK TSYLEPPPDG YENVTNIVPP YNAFSAQGMP EGDLVYVNYA RTEDFFKLER 190 200 210 220 230 240 EMGINCTGKI VIARYGKIFR GNKVKNAMLA GAIGIILYSD PADYFAPEVQ PYPKGWNLPG 250 260 270 280 290 300 TAAQRGNVLN LNGAGDPLTP GYPAKEYTFR LDVEEGVGIP RIPVHPIGYN DAEILLRYLG 310 320 330 340 350 360 GIAPPDKSWK GALNVSYSIG PGFTGSDSFR KVRMHVYNIN KITRIYNVVG TIRGSVEPDR 370 380 390 400 410 420 YVILGGHRDS WVFGAIDPTS GVAVLQEIAR SFGKLMSKGW RPRRTIIFAS WDAEEFGLLG 430 440 450 460 470 480 STEWAEENVK ILQERSIAYI NSDSSIEGNY TLRVDCTPLL YQLVYKLTKE IPSPDDGFES 490 500 510 520 530 540 KSLYESWLEK DPSPENKNLP RINKLGSGSD FEAYFQRLGI ASGRARYTKN KKTDKYSSYP 550 560 570 580 590 600 VYHTIYETFE LVEKFYDPTF KKQLSVAQLR GALVYELVDS KIIPFNIQDY AEALKNYAAS 610 620 630 640 650 660 IYNLSKKHDQ QLTDHGVSFD SLFSAVKNFS EAASDFHKRL IQVDLNNPIA VRMMNDQLML 670 680 690 700 710 720 LERAFIDPLG LPGKLFYRHI IFAPSSHNKY AGESFPGIYD AIFDIENKAN SRLAWKEVKK 730 HISIAAFTIQ AAAGTLKEVL

CATM prediction

The sequence of NALD2_HUMAN returned 4 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       10        20        30   
+    |    +    |    +    |    		 (Å) (°) (Å) (°)  
Model 1 RGRLYLWMCLAAALASFLMGFMVGWFIKAL 6.8 -55.0° 3.6 63.0 -38.8
Model 2 RGRLYLWMCLAAALASFLMGFMVGWFIKAL 7.3 -30.0° 4.7 38.9 -35.3
Model 3 RGRLYLWMCLAAALASFLMGFMVGWFIKAL 7.2 -45.0° 0.3 59.2 -26.5
Model 4 RGRLYLWMCLAAALASFLMGFMVGWFIKAL 7.0 -29.0° 2.6 59.7 -21.4
Prolines* RGRLYLWMCLAAALASFLMGFMVGWFIKPL          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 25, PHE, HA A, 24, GLY, O 2.90Å
B, 28, GLY, HA1 A, 25, PHE, O 3.16Å
A, 25, PHE, HA B, 24, GLY, O 2.90Å
A, 28, GLY, HA1 B, 25, PHE, O 3.16Å