7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

UniProt data

UniProt name: CP8B1_HUMAN (Q9UNU6)

Gene name: CYP8B1; Synonyms=CYP12

Organism: Homo sapiens (Human).

Function: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. {ECO:0000250|UniProtKB:O02766}.

Subcellular location: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

Transmembrane domains:

  1. 1-21, 21 AA, Helical. {ECO:0000255}.

Sequence: 501 AA

10 20 30 40 50 60 MVLWGPVLGA LLVVIAGYLC LPGMLRQRRP WEPPLDKGTV PWLGHAMAFR KNMFEFLKRM 70 80 90 100 110 120 RTKHGDVFTV QLGGQYFTFV MDPLSFGSIL KDTQRKLDFG QYAKKLVLKV FGYRSVQGDH 130 140 150 160 170 180 EMIHSASTKH LRGDGLKDLN ETMLDSLSFV MLTSKGWSLD ASCWHEDSLF RFCYYILFTA 190 200 210 220 230 240 GYLSLFGYTK DKEQDLLQAG ELFMEFRKFD LLFPRFVYSL LWPREWLEVG RLQRLFHKML 250 260 270 280 290 300 SVSHSQEKEG ISNWLGNMLQ FLREQGVPSA MQDKFNFMML WASQGNTGPT SFWALLYLLK 310 320 330 340 350 360 HPEAIRAVRE EATQVLGEAR LETKQSFAFK LGALQHTPVL DSVVEETLRL RAAPTLLRLV 370 380 390 400 410 420 HEDYTLKMSS GQEYLFRHGD ILALFPYLSV HMDPDIHPEP TVFKYDRFLN PNGSRKVDFF 430 440 450 460 470 480 KTGKKIHHYT MPWGSGVSIC PGRFFALSEV KLFILLMVTH FDLELVDPDT PLPHVDPQRW 490 500 GFGTMQPSHD VRFRYRLHPT E

CATM prediction

The sequence of CP8B1_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
           10        20   
    +    |    +    |    		 (Å) (°) (Å) (°)  
Model 1 MVLWGAVLGALLVVIAGYLCLAGM 6.7 -45.8° 4.6 38.0 -28.0
Model 2 MVLWGAVLGALLVVIAGYLCLAGM 6.9 -23.1° 4.2 54.0 -25.6
Model 3 MVLWGAVLGALLVVIAGYLCLAGM 6.6 -51.0° 2.4 44.9 -24.3
Prolines* MVLWGPVLGALLVVIAGYLCLPGM          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 5, GLY, HA1 A, 2, VAL, O 3.36Å
B, 6, ALA, HA A, 5, GLY, O 2.66Å
B, 9, GLY, HA1 A, 6, ALA, O 2.95Å
A, 5, GLY, HA1 B, 2, VAL, O 3.36Å
A, 6, ALA, HA B, 5, GLY, O 2.66Å
A, 9, GLY, HA1 B, 6, ALA, O 2.95Å