Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

UniProt data

UniProt name: MGT4A_HUMAN (Q9UM21)

Gene name: MGAT4A

Organism: Homo sapiens (Human).

Function: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N- linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.

Subcellular location: Alpha-1,3-mannosyl-glycoprotein 4-beta-N- acetylglucosaminyltransferase A soluble form: Secreted {ECO:0000250}.

Transmembrane domains:

  1. 5-27, 23 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 535 AA

10 20 30 40 50 60 MRLRNGTVAT ALAFITSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRI AEHRISQRSS 70 80 90 100 110 120 ELNTIVQQFK RVGAETNGSK DALNKFSDNT LKLLKELTSK KSLQVPSIYY HLPHLLKNEG 130 140 150 160 170 180 SLQPAVQIGN GRTGVSIVMG IPTVKREVKS YLIETLHSLI DNLYPEEKLD CVIVVFIGET 190 200 210 220 230 240 DIDYVHGVVA NLEKEFSKEI SSGLVEVISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL 250 260 270 280 290 300 DYCFLMMYAQ EKGIYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF 310 320 330 340 350 360 QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL RIRFRPSLFQ 370 380 390 400 410 420 HVGLHSSLSG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL KVYQGHTLEK TYMGEDFFWA 430 440 450 460 470 480 ITPIAGDYIL FKFDKPVNVE SYLFHSGNQE HPGDILLNTT VEVLPFKSEG LEISKETKDK 490 500 510 520 530 RLEDGYFRIG KFENGVAEGM VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKATN

CATM prediction

The sequence of MGT4A_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
          10        20        3
   +    |    +    |    +    |		 (Å) (°) (Å) (°)  
Model 1 RLRNGTVATALAFITSFLTLSWYTTWQNG 7.4 -50.0° 2.9 47.0 -8.0
Model 2 RLRNGTVATALAFITSFLTLSWYTTWQNG 7.0 -36.2° 3.6 36.2 -4.2

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 7, THR, HA A, 10, THR, OG1 2.76Å
A, 7, THR, HA B, 10, THR, OG1 2.76Å