CATM - Q9P2E5: Chondroitin sulfate glucuronyltransferase

UniProt data

Gene name: CHPF2; Synonyms=CHSY3, CSGLCAT, KIAA1402; ORFNames=UNQ299/PRO339

Organism: Homo sapiens (Human).

Function: Transfers glucuronic acid (GlcUA) from UDP-GlcUA to N- acetylgalactosamine residues on the non-reducing end of the elongating chondroitin polymer. Has no N- acetylgalactosaminyltransferase activity. {ECO:0000269|PubMed:12145278, ECO:0000269|PubMed:18316376}.

Subcellular location: Golgi apparatus, Golgi stack membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.

Transmembrane domains:

7-29, 23 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 772 AA

10 20 30 40 50 60 MRLSSLLALL RPALPLILGL SLGCSLSLLR VSWIQGEGED PCVEAVGERG GPQNPDSRAR 70 80 90 100 110 120 LDQSDEDFKP RIVPYYRDPN KPYKKVLRTR YIQTELGSRE RLLVAVLTSR ATLSTLAVAV 130 140 150 160 170 180 NRTVAHHFPR LLYFTGQRGA RAPAGMQVVS HGDERPAWLM SETLRHLHTH FGADYDWFFI 190 200 210 220 230 240 MQDDTYVQAP RLAALAGHLS INQDLYLGRA EEFIGAGEQA RYCHGGFGYL LSRSLLLRLR 250 260 270 280 290 300 PHLDGCRGDI LSARPDEWLG RCLIDSLGVG CVSQHQGQQY RSFELAKNRD PEKEGSSAFL 310 320 330 340 350 360 SAFAVHPVSE GTLMYRLHKR FSALELERAY SEIEQLQAQI RNLTVLTPEG EAGLSWPVGL 370 380 390 400 410 420 PAPFTPHSRF EVLGWDYFTE QHTFSCADGA PKCPLQGASR ADVGDALETA LEQLNRRYQP 430 440 450 460 470 480 RLRFQKQRLL NGYRRFDPAR GMEYTLDLLL ECVTQRGHRR ALARRVSLLR PLSRVEILPM 490 500 510 520 530 540 PYVTEATRVQ LVLPLLVAEA AAAPAFLEAF AANVLEPREH ALLTLLLVYG PREGGRGAPD 550 560 570 580 590 600 PFLGVKAAAA ELERRYPGTR LAWLAVRAEA PSQVRLMDVV SKKHPVDTLF FLTTVWTRPG 610 620 630 640 650 660 PEVLNRCRMN AISGWQAFFP VHFQEFNPAL SPQRSPPGPP GAGPDPPSPP GADPSRGAPI 670 680 690 700 710 720 GGRFDRQASA EGCFYNADYL AARARLAGEL AGQEEEEALE GLEVMDVFLR FSGLHLFRAV 730 740 750 760 770 EPGLVQKFSL RDCSPRLSEE LYHRCRLSNL EGLGGRAQLA MALFEQEQAN ST

CATM prediction

The sequence of CHPF2_HUMAN returned 5 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

#	Sequence and interface	d	θ	Z'	ω'	Score
	10 20 30 + \| + \| + \|	(Å)	(°)	(Å)	(°)
Model 1	SSLLALLRAALALILGLSLGCSLSLLRVS	6.9	-28.0°	3.2	48.0	-59.1
Model 2	SSLLALLRAALALILGLSLGCSLSLLRVS	6.4	-43.9°	0.9	46.0	-49.9
Model 3	SSLLALLRAALALILGLSLGCSLSLLRVS	7.2	-44.1°	5.0	43.9	-33.4
Model 4	SSLLALLRAALALILGLSLGCSLSLLRVS	6.8	-53.7°	2.8	51.0	-29.6
Model 5	SSLLALLRAALALILGLSLGCSLSLLRVS	7.1	-53.9°	2.6	32.5	-0.4
Prolines*	SSLLALLRPALPLILGLSLGCSLSLLRVS

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:

Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor	Acceptor	Distance
B, 16, LEU, HA	A, 15, ALA, O	2.76Å
B, 19, GLY, HA1	A, 16, LEU, O	3.13Å
B, 20, LEU, HA	A, 19, GLY, O	3.24Å
A, 16, LEU, HA	B, 15, ALA, O	2.76Å
A, 19, GLY, HA1	B, 16, LEU, O	3.13Å
A, 20, LEU, HA	B, 19, GLY, O	3.24Å

Donor	Acceptor	Distance
B, 20, LEU, HA	A, 19, GLY, O	2.85Å
B, 23, GLY, HA1	A, 20, LEU, O	2.81Å
A, 20, LEU, HA	B, 19, GLY, O	2.85Å
A, 23, GLY, HA1	B, 20, LEU, O	2.81Å
A, 24, CYS, HA	B, 27, SER, OG	3.39Å
A, 27, SER, HG1	B, 27, SER, OG	1.87Å

Donor	Acceptor	Distance
B, 23, GLY, HA1	A, 20, LEU, O	3.23Å
B, 24, CYS, HA	A, 23, GLY, O	3.28Å
B, 27, SER, HG1	A, 24, CYS, O	2.36Å
A, 23, GLY, HA1	B, 20, LEU, O	3.23Å
A, 24, CYS, HA	B, 23, GLY, O	3.28Å
A, 27, SER, HG1	B, 24, CYS, O	2.36Å

Donor	Acceptor	Distance
B, 16, LEU, HA	A, 15, ALA, O	3.17Å
B, 19, GLY, HA1	A, 16, LEU, O	2.67Å
B, 20, LEU, HA	A, 19, GLY, O	2.32Å
B, 23, GLY, HA1	A, 20, LEU, O	3.11Å
A, 16, LEU, HA	B, 15, ALA, O	3.17Å
A, 19, GLY, HA1	B, 16, LEU, O	2.67Å
A, 20, LEU, HA	B, 19, GLY, O	2.32Å
A, 23, GLY, HA1	B, 20, LEU, O	3.11Å

Donor	Acceptor	Distance
B, 16, LEU, HA	A, 15, ALA, O	3.05Å
B, 19, GLY, HA1	A, 16, LEU, O	3.48Å
A, 16, LEU, HA	B, 15, ALA, O	3.05Å
A, 19, GLY, HA1	B, 16, LEU, O	3.48Å