Stromal interaction molecule 2

UniProt data

UniProt name: STIM2_HUMAN (Q9P246)

Gene name: STIM2; Synonyms=KIAA1482

Organism: Homo sapiens (Human).

Function: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx. {ECO:0000269|PubMed:16005298, ECO:0000269|PubMed:16860747, ECO:0000269|PubMed:17905723, ECO:0000269|PubMed:18160041, ECO:0000269|PubMed:21217057, ECO:0000269|PubMed:22464749, ECO:0000269|PubMed:23359669}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:16860747, ECO:0000269|PubMed:17905723, ECO:0000269|PubMed:18160041}; Single-pass type I membrane protein {ECO:0000269|PubMed:16860747, ECO:0000269|PubMed:17905723, ECO:0000269|PubMed:18160041}. Note=Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration.

Transmembrane domains:

  1. 219-235, 17 AA, Helical. {ECO:0000255}.

Sequence: 746 AA

10 20 30 40 50 60 MLVLGLLVAG AADGCELVPR HLRGRRATGS AATAASSPAA AAGDSPALMT DPCMSLSPPC 70 80 90 100 110 120 FTEEDRFSLE ALQTIHKQMD DDKDGGIEVE ESDEFIREDM KYKDATNKHS HLHREDKHIT 130 140 150 160 170 180 IEDLWKRWKT SEVHNWTLED TLQWLIEFVE LPQYEKNFRD NNVKGTTLPR IAVHEPSFMI 190 200 210 220 230 240 SQLKISDRSH RQKLQLKALD VVLFGPLTRP PHNWMKDFIL TVSIVIGVGG CWFAYTQNKT 250 260 270 280 290 300 SKEHVAKMMK DLESLQTAEQ SLMDLQERLE KAQEENRNVA VEKQNLERKM MDEINYAKEE 310 320 330 340 350 360 ACRLRELREG AECELSRRQY AEQELEQVRM ALKKAEKEFE LRSSWSVPDA LQKWLQLTHE 370 380 390 400 410 420 VEVQYYNIKR QNAEMQLAIA KDEAEKIKKK RSTVFGTLHV AHSSSLDEVD HKILEAKKAL 430 440 450 460 470 480 SELTTCLRER LFRWQQIEKI CGFQIAHNSG LPSLTSSLYS DHSWVVMPRV SIPPYPIAGG 490 500 510 520 530 540 VDDLDEDTPP IVSQFPGTMA KPPGSLARSS SLCRSRRSIV PSSPQPQRAQ LAPHAPHPSH 550 560 570 580 590 600 PRHPHHPQHT PHSLPSPDPD ILSVSSCPAL YRNEEEEEAI YFSAEKQWEV PDTASECDSL 610 620 630 640 650 660 NSSIGRKQSP PLSLEIYQTL SPRKISRDEV SLEDSSRGDS PVTVDVSWGS PDCVGLTETK 670 680 690 700 710 720 SMIFSPASKV YNGILEKSCS MNQLSSGIPV PKPRHTSCSS AGNDSKPVQE APSVARISSI 730 740 PHDLCHNGEK SKKPSKIKSL FKKKSK

CATM prediction

The sequence of STIM2_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
      220       230      
    |    +    |    +   		 (Å) (°) (Å) (°)  
Model 1 KDFILTVSIVIGVGGCWFAYTQN 7.3 -37.2° 2.2 36.1 -19.3
Model 2 KDFILTVSIVIGVGGCWFAYTQN 7.3 -47.0° 1.4 44.8 -4.0
Model 3 KDFILTVSIVIGVGGCWFAYTQN 6.9 -55.1° 1.5 45.0 -2.7

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 230, GLY, HA1 A, 227, GLY, O 3.36Å
B, 231, CYS, HA A, 230, GLY, O 3.49Å
B, 234, ALA, HA A, 235, TYR, OH 3.32Å
A, 230, GLY, HA1 B, 227, GLY, O 3.36Å
A, 231, CYS, HA B, 230, GLY, O 3.49Å
A, 234, ALA, HA B, 235, TYR, OH 3.32Å