Vesicle-associated membrane protein-associated protein A

UniProt data

UniProt name: VAPA_HUMAN (Q9P0L0)

Gene name: VAPA; Synonyms=VAP33

Organism: Homo sapiens (Human).

Function: Binds to OSBPL3, which mediates recruitment of VAPA to plasma membrane sites (PubMed:25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed:25447204). With OSBPL3, may regulate ER morphology (PubMed:16143324). May play a role in vesicle trafficking (PubMed:11511104, PubMed:19289470). {ECO:0000269|PubMed:11511104, ECO:0000269|PubMed:16143324, ECO:0000269|PubMed:19289470, ECO:0000269|PubMed:25447204}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:10523508, ECO:0000269|PubMed:16143324, ECO:0000269|PubMed:19289470, ECO:0000269|PubMed:25447204}; Single- pass type IV membrane protein {ECO:0000269|PubMed:10523508, ECO:0000269|PubMed:19289470}. Cell membrane {ECO:0000269|PubMed:25447204}; Single-pass type IV membrane protein {ECO:0000305}. Cell junction, tight junction {ECO:0000269|PubMed:10523508}. Nucleus membrane {ECO:0000250|UniProtKB:Q9Z270}. Note=Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells. {ECO:0000269|PubMed:10523508}.

Transmembrane domains:

  1. 228-248, 21 AA, Helical; Anchor for type IV membrane protein. {ECO:0000255}.

Sequence: 249 AA

10 20 30 40 50 60 MASASGAMAK HEQILVLDPP TDLKFKGPFT DVVTTNLKLR NPSDRKVCFK VKTTAPRRYC 70 80 90 100 110 120 VRPNSGIIDP GSTVTVSVML QPFDYDPNEK SKHKFMVQTI FAPPNTSDME AVWKEAKPDE 130 140 150 160 170 180 LMDSKLRCVF EMPNENDKLN DMEPSKAVPL NASKQDGPMP KPHSVSLNDT ETRKLMEECK 190 200 210 220 230 240 RLQGEMMKLS EENRHLRDEG LRLRKVAHSD KPGSTSTASF RDNVTSPLPS LLVVIAAIFI GFFLGKFIL

CATM prediction

The sequence of VAPA_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       230       240       
+    |    +    |    +    		 (Å) (°) (Å) (°)  
Model 1 TSALASLLVVIAAIFIGFFLGKFIL 7.1 -51.0° 3.3 48.1 -27.6
Prolines* TSPLPSLLVVIAAIFIGFFLGKFIL          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 238, ILE, HA A, 237, ALA, O 3.01Å
B, 241, GLY, HA1 A, 238, ILE, O 3.07Å
A, 238, ILE, HA B, 237, ALA, O 3.01Å
A, 241, GLY, HA1 B, 238, ILE, O 3.07Å