Transmembrane prolyl 4-hydroxylase

UniProt data

UniProt name: P4HTM_HUMAN (Q9NXG6)

Gene name: P4HTM; Synonyms=PH4

Organism: Homo sapiens (Human).

Function: Catalyzes the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. {ECO:0000269|PubMed:17726031}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:12163023, ECO:0000269|PubMed:17726031}; Single-pass type II membrane protein {ECO:0000269|PubMed:12163023, ECO:0000269|PubMed:17726031}.

Transmembrane domains:

  1. 61-81, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 502 AA

10 20 30 40 50 60 MAAAAVTGQR PETAAAEEAS RPQWAPPDHC QAQAAAGLGD GEDAPVRPLC KPRGICSRAY 70 80 90 100 110 120 FLVLMVFVHL YLGNVLALLL FVHYSNGDES SDPGPQHRAQ GPGPEPTLGP LTRLEGIKVG 130 140 150 160 170 180 HERKVQLVTD RDHFIRTLSL KPLLFEIPGF LTDEECRLII HLAQMKGLQR SQILPTEEYE 190 200 210 220 230 240 EAMSTMQVSQ LDLFRLLDQN RDGHLQLREV LAQTRLGNGW WMTPESIQEM YAAIKADPDG 250 260 270 280 290 300 DGVLSLQEFS NMDLRDFHKY MRSHKAESSE LVRNSHHTWL YQGEGAHHIM RAIRQRVLRL 310 320 330 340 350 360 TRLSPEIVEL SEPLQVVRYG EGGHYHAHVD SGPVYPETIC SHTKLVANES VPFETSCRYM 370 380 390 400 410 420 TVLFYLNNVT GGGETVFPVA DNRTYDEMSL IQDDVDLRDT RRHCDKGNLR VKPQQGTAVF 430 440 450 460 470 480 WYNYLPDGQG WVGDVDDYSL HGGCLVTRGT KWIANNWINV DPSRARQALF QQEMARLARE 490 500 GGTDSQPEWA LDRAYRDARV EL

CATM prediction

The sequence of P4HTM_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
    60        70        80   
  |    +    |    +    |    		 (Å) (°) (Å) (°)  
Model 1 RAYFLVLMVFVHLYLGNVLALLLFVHY 7.0 -52.0° 1.3 57.1 -20.5

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 70, LEU, HA A, 69, HSE, O 3.47Å
B, 73, GLY, HA1 A, 70, LEU, O 3.12Å
B, 74, ASN, HA A, 73, GLY, O 3.49Å
A, 70, LEU, HA B, 69, HSE, O 3.47Å
A, 73, GLY, HA1 B, 70, LEU, O 3.12Å
A, 74, ASN, HA B, 73, GLY, O 3.49Å