Serine palmitoyltransferase 3

UniProt data

UniProt name: SPTC3_HUMAN (Q9NUV7)

Gene name: SPTLC3; Synonyms=C20orf38, SPTLC2L

Organism: Homo sapiens (Human).

Function: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference. {ECO:0000269|PubMed:19416851}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.

Transmembrane domains:

  1. 59-79, 21 AA, Helical. {ECO:0000255}.

Sequence: 552 AA

10 20 30 40 50 60 MANPGGGAVC NGKLHNHKKQ SNGSQSRNCT KNGIVKEAQQ NGKPHFYDKL IVESFEEAPL 70 80 90 100 110 120 HVMVFTYMGY GIGTLFGYLR DFLRNWGIEK CNAAVERKEQ KDFVPLYQDF ENFYTRNLYM 130 140 150 160 170 180 RIRDNWNRPI CSAPGPLFDL MERVSDDYNW TFRFTGRVIK DVINMGSYNF LGLAAKYDES 190 200 210 220 230 240 MRTIKDVLEV YGTGVASTRH EMGTLDKHKE LEDLVAKFLN VEAAMVFGMG FATNSMNIPA 250 260 270 280 290 300 LVGKGCLILS DELNHTSLVL GARLSGATIR IFKHNNTQSL EKLLRDAVIY GQPRTRRAWK 310 320 330 340 350 360 KILILVEGVY SMEGSIVHLP QIIALKKKYK AYLYIDEAHS IGAVGPTGRG VTEFFGLDPH 370 380 390 400 410 420 EVDVLMGTFT KSFGASGGYI AGRKDLVDYL RVHSHSAVYA SSMSPPIAEQ IIRSLKLIMG 430 440 450 460 470 480 LDGTTQGLQR VQQLAKNTRY FRQRLQEMGF IIYGNENASV VPLLLYMPGK VAAFARHMLE 490 500 510 520 530 540 KKIGVVVVGF PATPLAEARA RFCVSAAHTR EMLDTVLEAL DEMGDLLQLK YSRHKKSARP 550 ELYDETSFEL ED

CATM prediction

The sequence of SPTC3_HUMAN returned 5 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
      60        70        80 
    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 EEAALHVMVFTYMGYGIGTLFGYLRDF 6.4 -38.0° 2.9 61.0 -48.0
Model 2 EEAALHVMVFTYMGYGIGTLFGYLRDF 6.4 -51.9° 1.4 57.1 -24.2
Model 3 EEAALHVMVFTYMGYGIGTLFGYLRDF 7.2 -37.0° 2.3 35.6 -18.8
Model 4 EEAALHVMVFTYMGYGIGTLFGYLRDF 6.9 -53.3° 1.9 45.1 -17.7
Model 5 EEAALHVMVFTYMGYGIGTLFGYLRDF 7.1 -43.0° 0.0 33.6 -1.9
Prolines* EEAPLHVMVFTYMGYGIGTLFGYLRDF          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 69, GLY, HA1 A, 66, THR, O 3.24Å
B, 70, TYR, HA A, 69, GLY, O 2.43Å
B, 73, GLY, HA1 A, 70, TYR, O 2.83Å
B, 74, THR, HA A, 73, GLY, O 3.27Å
A, 69, GLY, HA1 B, 66, THR, O 3.24Å
A, 70, TYR, HA B, 69, GLY, O 2.43Å
A, 73, GLY, HA1 B, 70, TYR, O 2.83Å
A, 74, THR, HA B, 73, GLY, O 3.27Å