Mitochondrial dynamics protein MID51

UniProt data

UniProt name: MID51_HUMAN (Q9NQG6)

Gene name: MIEF1; Synonyms=MID51, SMCR7L

Organism: Homo sapiens (Human).

Function: Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L. {ECO:0000269|PubMed:21508961, ECO:0000269|PubMed:21701560, ECO:0000269|PubMed:23283981, ECO:0000269|PubMed:23530241, ECO:0000269|PubMed:23921378, ECO:0000269|PubMed:24515348}.

Subcellular location: Mitochondrion outer membrane {ECO:0000269|PubMed:21508961, ECO:0000269|PubMed:21701560, ECO:0000269|PubMed:24515348}; Single-pass membrane protein {ECO:0000269|PubMed:21508961, ECO:0000269|PubMed:21701560, ECO:0000269|PubMed:24515348}.

Transmembrane domains:

  1. 24-46, 23 AA, Helical. {ECO:0000255}.

Sequence: 463 AA

10 20 30 40 50 60 MAGAGERKGK KDDNGIGTAI DFVLSNARLV LGVGGAAMLG IATLAVKRMY DRAISAPTSP 70 80 90 100 110 120 TRLSHSGKRS WEEPNWMGSP RLLNRDMKTG LSRSLQTLPT DSSTFDTDTF CPPRPKPVAR 130 140 150 160 170 180 KGQVDLKKSR LRMSLQEKLL TYYRNRAAIP AGEQARAKQA AVDICAELRS FLRAKLPDMP 190 200 210 220 230 240 LRDMYLSGSL YDDLQVVTAD HIQLIVPLVL EQNLWSCIPG EDTIMNVPGF FLVRRENPEY 250 260 270 280 290 300 FPRGSSYWDR CVVGGYLSPK TVADTFEKVV AGSINWPAIG SLLDYVIRPA PPPEALTLEV 310 320 330 340 350 360 QYERDKHLFI DFLPSVTLGD TVLVAKPHRL AQYDNLWRLS LRPAETARLR ALDQADSGCR 370 380 390 400 410 420 SLCLKILKAI CKSTPALGHL TASQLTNVIL HLAQEEADWS PDMLADRFLQ ALRGLISYLE 430 440 450 460 AGVLPSALNP KVNLFAELTP EEIDELGYTL YCSLSEPEVL LQT

CATM prediction

The sequence of MID51_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
           30        40        
    +    |    +    |    +    		 (Å) (°) (Å) (°)  
Model 1 DFVLSNARLVLGVGGAAMLGIATLAVKRM 7.3 -50.9° 2.4 45.2 -10.5
Model 2 DFVLSNARLVLGVGGAAMLGIATLAVKRM 7.4 -32.9° 2.2 35.2 -7.8
Model 3 DFVLSNARLVLGVGGAAMLGIATLAVKRM 7.1 -53.8° 4.8 40.9 -0.5

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 32, GLY, HA1 A, 29, LEU, O 3.13Å
B, 33, VAL, HA A, 32, GLY, O 3.44Å
A, 32, GLY, HA1 B, 29, LEU, O 3.13Å
A, 33, VAL, HA B, 32, GLY, O 3.44Å