Calcium-independent phospholipase A2-gamma

UniProt data

UniProt name: PLPL8_HUMAN (Q9NP80)

Gene name: PNPLA8; Synonyms=IPLA22, IPLA2G; ORFNames=BM-043

Organism: Homo sapiens (Human).

Function: Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids. {ECO:0000269|PubMed:10744668, ECO:0000269|PubMed:15695510}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}.

Transmembrane domains:

  1. 475-495, 21 AA, Helical. {ECO:0000255}.

Sequence: 782 AA

10 20 30 40 50 60 MSINLTVDIY IYLLSNARSV CGKQRSKQLY FLFSPKHYWR ISHISLQRGF HTNIIRCKWT 70 80 90 100 110 120 KSEAHSCSKH CYSPSNHGLH IGILKLSTSA PKGLTKVNIC MSRIKSTLNS VSKAVFGNQN 130 140 150 160 170 180 EMISRLAQFK PSSQILRKVS DSGWLKQKNI KQAIKSLKKY SDKSAEKSPF PEEKSHIIDK 190 200 210 220 230 240 EEDIGKRSLF HYTSSITTKF GDSFYFLSNH INSYFKRKEK MSQQKENEHF RDKSELEDKK 250 260 270 280 290 300 VEEGKLRSPD PGILAYKPGS ESVHTVDKPT SPSAIPDVLQ VSTKQSIANF LSRPTEGVQA 310 320 330 340 350 360 LVGGYIGGLV PKLKYDSKSQ SEEQEEPAKT DQAVSKDRNA EEKKRLSLQR EKIIARVSID 370 380 390 400 410 420 NRTRALVQAL RRTTDPKLCI TRVEELTFHL LEFPEGKGVA VKERIIPYLL RLRQIKDETL 430 440 450 460 470 480 QAAVREILAL IGYVDPVKGR GIRILSIDGG GTRGVVALQT LRKLVELTQK PVHQLFDYIC 490 500 510 520 530 540 GVSTGAILAF MLGLFHMPLD ECEELYRKLG SDVFSQNVIV GTVKMSWSHA FYDSQTWENI 550 560 570 580 590 600 LKDRMGSALM IETARNPTCP KVAAVSTIVN RGITPKAFVF RNYGHFPGIN SHYLGGCQYK 610 620 630 640 650 660 MWQAIRASSA APGYFAEYAL GNDLHQDGGL LLNNPSALAM HECKCLWPDV PLECIVSLGT 670 680 690 700 710 720 GRYESDVRNT VTYTSLKTKL SNVINSATDT EEVHIMLDGL LPPDTYFRFN PVMCENIPLD 730 740 750 760 770 780 ESRNEKLDQL QLEGLKYIER NEQKMKKVAK ILSQEKTTLQ KINDWIKLKT DMYEGLPFFS KL

CATM prediction

The sequence of PLPL8_HUMAN returned 5 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
          480       490      
   +    |    +    |    +   		 (Å) (°) (Å) (°)  
Model 1 VHQLFDYICGVSTGAILAFMLGLFHMA 6.5 -43.0° 5.4 59.9 -49.0
Model 2 VHQLFDYICGVSTGAILAFMLGLFHMA 6.7 -46.8° 2.2 45.2 -39.4
Model 3 VHQLFDYICGVSTGAILAFMLGLFHMA 7.3 -26.0° 2.0 48.8 -31.7
Model 4 VHQLFDYICGVSTGAILAFMLGLFHMA 7.4 -24.9° 1.2 49.0 -29.9
Model 5 VHQLFDYICGVSTGAILAFMLGLFHMA 7.0 -55.0° 2.4 47.9 -22.3
Prolines* VHQLFDYICGVSTGAILAFMLGLFHMP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 478, TYR, HA A, 477, ASP, O 3.25Å
B, 481, GLY, HA1 A, 478, TYR, O 2.87Å
B, 482, VAL, HA A, 481, GLY, O 2.56Å
B, 485, GLY, HA1 A, 482, VAL, O 3.40Å
B, 478, TYR, HH A, 484, THR, OG1 2.51Å
A, 478, TYR, HA B, 477, ASP, O 3.25Å
A, 481, GLY, HA1 B, 478, TYR, O 2.87Å
A, 482, VAL, HA B, 481, GLY, O 2.56Å
A, 485, GLY, HA1 B, 482, VAL, O 3.40Å
A, 478, TYR, HH B, 484, THR, OG1 2.51Å