Roundabout homolog 2

UniProt data

UniProt name: ROBO2_HUMAN (Q9HCK4)

Gene name: ROBO2; Synonyms=KIAA1568

Organism: Homo sapiens (Human).

Function: Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.

Subcellular location: Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 860-880, 21 AA, Helical. {ECO:0000255}.

Sequence: 1378 AA

10 20 30 40 50 60 MSLLMFTQLL LCGFLYVRVD GSRLRQEDFP PRIVEHPSDV IVSKGEPTTL NCKAEGRPTP 70 80 90 100 110 120 TIEWYKDGER VETDKDDPRS HRMLLPSGSL FFLRIVHGRR SKPDEGSYVC VARNYLGEAV 130 140 150 160 170 180 SRNASLEVAL LRDDFRQNPT DVVVAAGEPA ILECQPPRGH PEPTIYWKKD KVRIDDKEER 190 200 210 220 230 240 ISIRGGKLMI SNTRKSDAGM YTCVGTNMVG ERDSDPAELT VFERPTFLRR PINQVVLEEE 250 260 270 280 290 300 AVEFRCQVQG DPQPTVRWKK DDADLPRGRY DIKDDYTLRI KKTMSTDEGT YMCIAENRVG 310 320 330 340 350 360 KMEASATLTV RAPPQFVVRP RDQIVAQGRT VTFPCETKGN PQPAVFWQKE GSQNLLFPNQ 370 380 390 400 410 420 PQQPNSRCSV SPTGDLTITN IQRSDAGYYI CQALTVAGSI LAKAQLEVTD VLTDRPPPII 430 440 450 460 470 480 LQGPANQTLA VDGTALLKCK ATGDPLPVIS WLKEGFTFPG RDPRATIQEQ GTLQIKNLRI 490 500 510 520 530 540 SDTGTYTCVA TSSSGETSWS AVLDVTESGA TISKNYDLSD LPGPPSKPQV TDVTKNSVTL 550 560 570 580 590 600 SWQPGTPGTL PASAYIIEAF SQSVSNSWQT VANHVKTTLY TVRGLRPNTI YLFMVRAINP 610 620 630 640 650 660 QGLSDPSPMS DPVRTQDISP PAQGVDHRQV QKELGDVLVR LHNPVVLTPT TVQVTWTVDR 670 680 690 700 710 720 QPQFIQGYRV MYRQTSGLQA TSSWQNLDAK VPTERSAVLV NLKKGVTYEI KVRPYFNEFQ 730 740 750 760 770 780 GMDSESKTVR TTEEAPSAPP QSVTVLTVGS YNSTSISVSW DPPPPDHQNG IIQEYKIWCL 790 800 810 820 830 840 GNETRFHINK TVDAAIRSVI IGGLFPGIQY RVEVAASTSA GVGVKSEPQP IIIGRRNEVV 850 860 870 880 890 900 ITENNNSITE QITDVVKQPA FIAGIGGACW VILMGFSIWL YWRRKKRKGL SNYAVTFQRG 910 920 930 940 950 960 DGGLMSNGSR PGLLNAGDPS YPWLADSWPA TSLPVNNSNS GPNEIGNFGR GDVLPPVPGQ 970 980 990 1000 1010 1020 GDKTATMLSD GAIYSSIDFT TKTSYNSSSQ ITQATPYATT QILHSNSIHE LAVDLPDPQW 1030 1040 1050 1060 1070 1080 KSSIQQKTDL MGFGYSLPDQ NKGNNGGKGG KKKKNKNSSK PQKNNGSTWA NVPLPPPPVQ 1090 1100 1110 1120 1130 1140 PLPGTELEHY AVEQQENGYD SDSWCPPLPV QTYLHQGLED ELEEDDDRVP TPPVRGVASS 1150 1160 1170 1180 1190 1200 PAISFGQQST ATLTPSPREE MQPMLQAHLD ELTRAYQFDI AKQTWHIQSN NQPPQPPVPP 1210 1220 1230 1240 1250 1260 LGYVSGALIS DLETDVADDD ADDEEEALEI PRPLRALDQT PGSSMDNLDS SVTGKAFTSS 1270 1280 1290 1300 1310 1320 QRPRPTSPFS TDSNTSAALS QSQRPRPTKK HKGGRMDQQP ALPHRREGMT DEEALVPYSK 1330 1340 1350 1360 1370 PSFPSPGGHS SSGTASSKGS TGPRKTEVLR AGHQRNASDL LDIGYMGSNS QGQFTGEL

CATM prediction

The sequence of ROBO2_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
     860       870       880 
   |    +    |    +    |   		 (Å) (°) (Å) (°)  
Model 1 KQAAFIAGIGGACWVILMGFSIWLYWR 7.0 -43.2° 4.5 34.9 -25.1
Model 2 KQAAFIAGIGGACWVILMGFSIWLYWR 7.0 -55.0° 2.4 47.9 -24.0
Model 3 KQAAFIAGIGGACWVILMGFSIWLYWR 7.1 -54.0° 2.5 33.0 -10.8
Prolines* KQPAFIAGIGGACWVILMGFSIWLYWR          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 864, GLY, HA2 A, 863, ALA, O 2.97Å
B, 867, GLY, HA1 A, 864, GLY, O 3.15Å
A, 864, GLY, HA2 B, 863, ALA, O 2.97Å
A, 867, GLY, HA1 B, 864, GLY, O 3.15Å