Protein amnionless

UniProt data

UniProt name: AMNLS_HUMAN (Q9BXJ7)

Gene name: AMN; ORFNames=UNQ513/PRO1028

Organism: Homo sapiens (Human).

Function: Necessary for efficient absorption of vitamin B12 (PubMed:12590260, PubMed:14576052). Required for normal CUBN- mediated protein transport in the kidney. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm (By similarity). {ECO:0000250|UniProtKB:Q99JB7, ECO:0000269|PubMed:14576052, ECO:0000305|PubMed:12590260}.

Subcellular location: Apical cell membrane {ECO:0000250|UniProtKB:Q99JB7}; Single-pass type I membrane protein {ECO:0000305}. Cell membrane {ECO:0000269|PubMed:14576052}; Peripheral membrane protein {ECO:0000305}. Endosome {ECO:0000305|PubMed:14576052}. Membrane, coated pit {ECO:0000305|PubMed:14576052}.

Transmembrane domains:

  1. 358-378, 21 AA, Helical. {ECO:0000255}.

Sequence: 453 AA

10 20 30 40 50 60 MGVLGRVLLW LQLCALTQAV SKLWVPNTDF DVAANWSQNR TPCAGGAVEF PADKMVSVLV 70 80 90 100 110 120 QEGHAVSDML LPLDGELVLA SGAGFGVSDV GSHLDCGAGE PAVFRDSDRF SWHDPHLWRS 130 140 150 160 170 180 GDEAPGLFFV DAERVPCRHD DVFFPPSASF RVGLGPGASP VRVRSISALG RTFTRDEDLA 190 200 210 220 230 240 VFLASRAGRL RFHGPGALSV GPEDCADPSG CVCGNAEAQP WICAALLQPL GGRCPQAACH 250 260 270 280 290 300 SALRPQGQCC DLCGAVVLLT HGPAFDLERY RARILDTFLG LPQYHGLQVA VSKVPRSSRL 310 320 330 340 350 360 READTEIQVV LVENGPETGG AGRLARALLA DVAENGEALG VLEATMRESG AHVWGSSAAG 370 380 390 400 410 420 LAGGVAAAVL LALLVLLVAP PLLRRAGRLR WRRHEAAAPA GAPLGFRNPV FDVTASEELP 430 440 450 LPRRLSLVPK AAADSTSHSY FVNPLFAGAE AEA

CATM prediction

The sequence of AMNLS_HUMAN returned 6 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       360       370       38
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 GSSAAGLAGGVAAAVLLALLVLLVAAA 6.9 -36.9° -0.0 37.3 -21.7
Model 2 GSSAAGLAGGVAAAVLLALLVLLVAAA 6.9 -23.0° 4.2 54.0 -14.6
Model 3 GSSAAGLAGGVAAAVLLALLVLLVAAA 6.9 -45.0° 4.0 39.4 -9.0
Model 4 GSSAAGLAGGVAAAVLLALLVLLVAAA 6.4 -48.0° 0.8 54.0 -7.7
Model 5 GSSAAGLAGGVAAAVLLALLVLLVAAA 7.2 -51.1° 1.2 44.9 -4.3
Model 6 GSSAAGLAGGVAAAVLLALLVLLVAAA 7.3 -53.9° 3.6 48.6 -3.6
Prolines* GSSAAGLAGGVAAAVLLALLVLLVAPP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 361, LEU, HA A, 360, GLY, O 2.81Å
B, 364, GLY, HA1 A, 361, LEU, O 3.29Å
A, 361, LEU, HA B, 360, GLY, O 2.81Å
A, 364, GLY, HA1 B, 361, LEU, O 3.29Å