Adenosine monophosphate-protein transferase FICD

UniProt data

UniProt name: FICD_HUMAN (Q9BVA6)

Gene name: FICD; Synonyms=HIP13, HYPE; ORFNames=UNQ3041/PRO9857

Organism: Homo sapiens (Human).

Function: Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified. {ECO:0000269|PubMed:19362538, ECO:0000269|PubMed:22266942}.

Subcellular location: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 24-44, 21 AA, Helical. {ECO:0000255}.

Sequence: 458 AA

10 20 30 40 50 60 MMLIPMASVM AVTEPKWVSV WSRFLWVTLL SMVLGSLLAL LLPLGAVEEQ CLAVLKGLYL 70 80 90 100 110 120 LRSKPDRAQH AATKCTSPST ELSITSRGAT LLVAKTKASP AGKLEARAAL NQALEMKRQG 130 140 150 160 170 180 KREKAQKLFM HALKMDPDFV DALTEFGIFS EEDKDIIQAD YLYTRALTIS PYHEKALVNR 190 200 210 220 230 240 DRTLPLVEEI DQRYFSIIDS KVKKVMSIPK GNSALRRVME ETYYHHIYHT VAIEGNTLTL 250 260 270 280 290 300 SEIRHILETR YAVPGKSLEE QNEVIGMHAA MKYINTTLVS RIGSVTISDV LEIHRRVLGY 310 320 330 340 350 360 VDPVEAGRFR TTQVLVGHHI PPHPQDVEKQ MQEFVQWLNS EEAMNLHPVE FAALAHYKLV 370 380 390 400 410 420 YIHPFIDGNG RTSRLLMNLI LMQAGYPPIT IRKEQRSDYY HVLEAANEGD VRPFIRFIAK 430 440 450 CTETTLDTLL FATTEYSVAL PEAQPNHSGF KETLPVKP

CATM prediction

The sequence of FICD_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
           30        40      
    +    |    +    |    +  		 (Å) (°) (Å) (°)  
Model 1 WSRFLWVTLLSMVLGSLLALLLALGAV 7.0 -38.0° 3.2 34.9 -35.2
Model 2 WSRFLWVTLLSMVLGSLLALLLALGAV 7.2 -55.0° 0.3 45.0 -4.6
Prolines* WSRFLWVTLLSMVLGSLLALLLPLGAV          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 28, THR, HA A, 31, SER, OG 3.30Å
B, 32, MET, HA A, 31, SER, O 3.10Å
B, 35, GLY, HA1 A, 32, MET, O 3.03Å
B, 36, SER, HA A, 35, GLY, O 3.37Å
A, 32, MET, HA B, 31, SER, O 3.10Å
A, 35, GLY, HA1 B, 32, MET, O 3.03Å
A, 36, SER, HA B, 35, GLY, O 3.37Å