Galactose-3-O-sulfotransferase 4

UniProt data

UniProt name: G3ST4_HUMAN (Q96RP7)

Gene name: GAL3ST4; ORFNames=PP6968

Organism: Homo sapiens (Human).

Function: Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc. {ECO:0000269|PubMed:11333265}.

Subcellular location: Golgi apparatus, Golgi stack membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 19-39, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 486 AA

10 20 30 40 50 60 MGPLSPARTL RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRQ PSAPSLRPAL 70 80 90 100 110 120 PSCPPRQRLV FLKTHKSGSS SVLSLLHRYG DQHGLRFALP ARYQFGYPKL FQASRVKGYR 130 140 150 160 170 180 PQGGGTQLPF HILCHHMRFN LKEVLQVMPS DSFFFSIVRD PAALARSAFS YYKSTSSAFR 190 200 210 220 230 240 KSPSLAAFLA NPRGFYRPGA RGDHYARNLL WFDFGLPFPP EKRAKRGNIH PPRDPNPPQL 250 260 270 280 290 300 QVLPSGAGPR AQTLNPNALI HPVSTVTDHR SQISSPASFD LGSSSFIQWG LAWLDSVFDL 310 320 330 340 350 360 VMVAEYFDES LVLLADALCW GLDDVVGFMH NAQAGHKQGL STVSNSGLTA EDRQLTARAR 370 380 390 400 410 420 AWNNLDWALY VHFNRSLWAR IEKYGQGRLQ TAVAELRARR EALAKHCLVG GEASDPKYIT 430 440 450 460 470 480 DRRFRPFQFG SAKVLGYILR SGLSPQDQEE CERLATPELQ YKDKLDAKQF PPTVSLPLKT SRPLSP

CATM prediction

The sequence of G3ST4_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
      20        30        40 
    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 RSLGVALGVFMTIGFALQLLGGAFQRR 6.8 -47.0° 1.3 45.2 -23.0
Model 2 RSLGVALGVFMTIGFALQLLGGAFQRR 6.4 -51.2° 4.8 56.9 -6.9
Prolines* RSLGVALGVFMTIGFALQLLGGPFQRR          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 33, GLN, HA A, 32, LEU, O 3.20Å
B, 36, GLY, HA1 A, 33, GLN, O 2.86Å
B, 37, GLY, HA2 A, 36, GLY, O 3.20Å
A, 33, GLN, HA B, 32, LEU, O 3.20Å
A, 36, GLY, HA1 B, 33, GLN, O 2.86Å
A, 37, GLY, HA2 B, 36, GLY, O 3.20Å