Roundabout homolog 3

UniProt data

UniProt name: ROBO3_HUMAN (Q96MS0)

Gene name: ROBO3

Organism: Homo sapiens (Human).

Function: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing. {ECO:0000250, ECO:0000269|PubMed:15105459}.

Subcellular location: Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 892-912, 21 AA, Helical. {ECO:0000255}.

Sequence: 1386 AA

10 20 30 40 50 60 MLRYLLKTLL QMNLFADSLA GDISNSSELL LGFNSSLAAL NHTLLPPGDP SLNGSRVGPE 70 80 90 100 110 120 DAMPRIVEQP PDLLVSRGEP ATLPCRAEGR PRPNIEWYKN GARVATVRED PRAHRLLLPS 130 140 150 160 170 180 GALFFPRIVH GRRARPDEGV YTCVARNYLG AAASRNASLE VAVLRDDFRQ SPGNVVVAVG 190 200 210 220 230 240 EPAVLECVPP RGHPEPSVSW RKDGARLKEE EGRITIRGGK LMMSHTLKSD AGMYVCVASN 250 260 270 280 290 300 MAGERESAAA EVMVLERPSF LRRPVNQVVL ADAPVTFLCE VKGDPPPRLR WRKEDGELPT 310 320 330 340 350 360 GRYEIRSDHS LWIGHVSAED EGTYTCVAEN SVGRAEASGS LSVHVPPQLV TQPQDQMAAP 370 380 390 400 410 420 GESVAFQCET KGNPPPAIFW QKEGSQVLLF PSQSLQPTGR FSVSPRGQLN ITAVQRGDAG 430 440 450 460 470 480 YYVCQAVSVA GSILAKALLE IKGASLDGLP PVILQGPANQ TLVLGSSVWL PCRVTGNPQP 490 500 510 520 530 540 SVRWKKDGQW LQGDDLQFKT MANGTLYIAN VQEMDMGFYS CVAKSSTGEA TWSGWLKMRE 550 560 570 580 590 600 DWGVSPDPPT EPSSPPGAPS QPVVTEITKN SITLTWKPNP QTGAAVTSYV IEAFSPAAGN 610 620 630 640 650 660 TWRTVADGVQ LETHTVSGLQ PNTIYLFLVR AVGAWGLSEP SPVSEPVRTQ DSSPSRPVED 670 680 690 700 710 720 PWRGQQGLAE VAVRLQEPIV LGPRTLQVSW TVDGPVQLVQ GFRVSWRVAG PEGGSWTMLD 730 740 750 760 770 780 LQSPSQQSTV LRGLPPGTQI QIKVQAQGQE GLGAESLSVT RSIPEEAPSG PPQGVAVALG 790 800 810 820 830 840 GDGNSSITVS WEPPLPSQQN GVITEYQIWC LGNESRFHLN RSAAGWARSA MLRGLVPGLL 850 860 870 880 890 900 YRTLVAAATS AGVGVPSAPV LVQLPSPPDL EPGLEVGAGL AVRLARVLRE PAFLAGSGAA 910 920 930 940 950 960 CGALLLGLCA ALYWRRKQRK ELSHYTASFA YTPAVSFPHS EGLSGASSRP PMGLGPAPYS 970 980 990 1000 1010 1020 WLADSWPHPS RSPSAQEPRG SCCPSNPDPD DRYYNEAGIS LYLAQTARGT AAPGEGPVYS 1030 1040 1050 1060 1070 1080 TIDPAGEELQ TFHGGFPQHP SGDLGPWSQY APPEWSQGDS GAKGGKVKLL GKPVQMPSLN 1090 1100 1110 1120 1130 1140 WPEALPPPPP SCELSCLEGP EEELEGSSEP EEWCPPMPER SHLTEPSSSG GCLVTPSRRE 1150 1160 1170 1180 1190 1200 TPSPTPSYGQ QSTATLTPSP PDPPQPPTDM PHLHQMPRRV PLGPSSPLSV SQPMLGIREA 1210 1220 1230 1240 1250 1260 RPAGLGAGPA ASPHLSPSPA PSTASSAPGR TWQGNGEMTP PLQGPRARFR KKPKALPYRR 1270 1280 1290 1300 1310 1320 ENSPGDLPPP PLPPPEEEAS WALELRAAGS MSSLERERSG ERKAVQAVPL AAQRVLHPDE 1330 1340 1350 1360 1370 1380 EAWLPYSRPS FLSRGQGTST CSTAGSNSSR GSSSSRGSRG PGRSRSRSQS RSQSQRPGQK RREEPR

CATM prediction

The sequence of ROBO3_HUMAN returned 7 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
   890       900       910   
 |    +    |    +    |    +		 (Å) (°) (Å) (°)  
Model 1 REAAFLAGSGAACGALLLGLCAALYWR 7.3 -29.0° 2.5 37.0 -28.4
Model 2 REAAFLAGSGAACGALLLGLCAALYWR 7.1 -39.8° 4.5 44.0 -23.3
Model 3 REAAFLAGSGAACGALLLGLCAALYWR 7.2 -42.0° 2.8 49.5 -22.7
Model 4 REAAFLAGSGAACGALLLGLCAALYWR 6.6 -48.0° 0.0 37.9 -18.7
Model 5 REAAFLAGSGAACGALLLGLCAALYWR 7.2 -36.9° 2.3 36.0 -15.0
Model 6 REAAFLAGSGAACGALLLGLCAALYWR 6.9 -53.2° 2.0 44.7 -7.1
Model 7 REAAFLAGSGAACGALLLGLCAALYWR 7.2 -37.0° 2.2 36.0 -2.7
Prolines* REPAFLAGSGAACGALLLGLCAALYWR          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 900, ALA, HA A, 899, ALA, O 3.49Å
B, 904, LEU, HA A, 903, ALA, O 3.32Å
A, 900, ALA, HA B, 899, ALA, O 3.49Å
A, 904, LEU, HA B, 903, ALA, O 3.32Å