Serine/threonine-protein phosphatase PGAM5, mitochondrial

UniProt data

UniProt name: PGAM5_HUMAN (Q96HS1)

Gene name: PGAM5

Organism: Homo sapiens (Human).

Function: Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2- dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore. {ECO:0000269|PubMed:18387606, ECO:0000269|PubMed:19590015, ECO:0000269|PubMed:22265414}.

Subcellular location: Mitochondrion outer membrane {ECO:0000269|PubMed:18387606}; Single-pass membrane protein {ECO:0000269|PubMed:18387606}. Note=Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.

Transmembrane domains:

  1. 7-29, 23 AA, Helical. {ECO:0000255}.

Sequence: 289 AA

10 20 30 40 50 60 MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRAGGDAEPR PAEPPAWAGG ARPGPGVWDP 70 80 90 100 110 120 NWDRREPLSL INVRKRNVES GEEELASKLD HYKAKATRHI FLIRHSQYHV DGSLEKDRTL 130 140 150 160 170 180 TPLGREQAEL TGLRLASLGL KFNKIVHSSM TRAIETTDII SRHLPGVCKV STDLLREGAP 190 200 210 220 230 240 IEPDPPVSHW KPEAVQYYED GARIEAAFRN YIHRADARQE EDSYEIFICH ANVIRYIVCR 250 260 270 280 ALQFPPEGWL RLSLNNGSIT HLVIRPNGRV ALRTLGDTGF MPPDKITRS

CATM prediction

The sequence of PGAM5_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
        10        20        30 
 +    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 RQALQLAACGLAGGSAAVLFSAVAVGKAR 6.8 -42.7° 3.0 34.9 -26.0
Model 2 RQALQLAACGLAGGSAAVLFSAVAVGKAR 6.8 -47.1° 1.3 45.0 -17.8
Model 3 RQALQLAACGLAGGSAAVLFSAVAVGKAR 7.4 -49.9° 2.9 46.8 -3.8
Prolines* RQALQLAACGLAGGSAAVLFSAVAVGKPR          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 13, GLY, HA2 A, 12, CYS, O 2.95Å
B, 16, GLY, HA1 A, 13, GLY, O 2.76Å
B, 17, GLY, HA2 A, 16, GLY, O 3.10Å
A, 13, GLY, HA2 B, 12, CYS, O 2.95Å
A, 16, GLY, HA1 B, 13, GLY, O 2.76Å
A, 17, GLY, HA2 B, 16, GLY, O 3.10Å