Atypical kinase COQ8B, mitochondrial {ECO:0000305}

UniProt data

UniProt name: COQ8B_HUMAN (Q96D53)

Gene name: COQ8B {ECO:0000312|HGNC:HGNC:19041}

Organism: Homo sapiens (Human).

Function: Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration (PubMed:24270420). Its substrate specificity is unclear: does not show any protein kinase activity. Probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway. Required for podocyte migration (PubMed:24270420). {ECO:0000250|UniProtKB:Q8NI60, ECO:0000269|PubMed:24270420}.

Subcellular location: Mitochondrion membrane {ECO:0000269|PubMed:24270420}; Single-pass membrane protein {ECO:0000269|PubMed:24270420}. Cytoplasm, cytosol {ECO:0000269|PubMed:24270420}. Cell membrane {ECO:0000269|PubMed:24270420}.

Transmembrane domains:

  1. 92-108, 17 AA, Helical. {ECO:0000255}.

Sequence: 544 AA

10 20 30 40 50 60 MWLKVGGLLR GTGGQLGQTV GWPCGALGPG PHRWGPCGGS WAQKFYQDGP GRGLGEEDIR 70 80 90 100 110 120 RAREARPRKT PRPQLSDRSR ERKVPASRIS RLANFGGLAV GLGLGVLAEM AKKSMPGGRL 130 140 150 160 170 180 QSEGGSGLDS SPFLSEANAE RIVQTLCTVR GAALKVGQML SIQDNSFISP QLQHIFERVR 190 200 210 220 230 240 QSADFMPRWQ MLRVLEEELG RDWQAKVASL EEVPFAAASI GQVHQGLLRD GTEVAVKIQY 250 260 270 280 290 300 PGIAQSIQSD VQNLLAVLKM SAALPAGLFA EQSLQALQQE LAWECDYRRE AACAQNFRQL 310 320 330 340 350 360 LANDPFFRVP AVVKELCTTR VLGMELAGGV PLDQCQGLSQ DLRNQICFQL LTLCLRELFE 370 380 390 400 410 420 FRFMQTDPNW ANFLYDASSH QVTLLDFGAS REFGTEFTDH YIEVVKAAAD GDRDCVLQKS 430 440 450 460 470 480 RDLKFLTGFE TKAFSDAHVE AVMILGEPFA TQGPYDFGSG ETARRIQDLI PVLLRHRLCP 490 500 510 520 530 540 PPEETYALHR KLAGAFLACA HLRAHIACRD LFQDTYHRYW ASRQPDAATA GSLPTKGDSW VDPS

CATM prediction

The sequence of COQ8B_HUMAN returned 8 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
   90        100       11
 |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 ISRLANFGGLAVGLGLGVLAEMA 6.7 -28.0° 3.1 48.2 -53.4
Model 2 ISRLANFGGLAVGLGLGVLAEMA 6.9 -27.9° 3.2 48.0 -49.2
Model 3 ISRLANFGGLAVGLGLGVLAEMA 6.9 -36.9° 0.0 36.7 -30.0
Model 4 ISRLANFGGLAVGLGLGVLAEMA 6.7 -43.1° 5.4 59.8 -20.3
Model 5 ISRLANFGGLAVGLGLGVLAEMA 6.6 -45.0° 0.2 44.9 -17.6
Model 6 ISRLANFGGLAVGLGLGVLAEMA 7.3 -48.0° 4.3 35.0 -6.3
Model 7 ISRLANFGGLAVGLGLGVLAEMA 7.1 -33.1° 2.3 34.8 -5.6
Model 8 ISRLANFGGLAVGLGLGVLAEMA 7.1 -51.0° 2.4 45.1 -2.8

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 94, ASN, HA A, 94, ASN, OD1 3.11Å
B, 97, GLY, HA1 A, 94, ASN, OD1 2.40Å
B, 97, GLY, HA1 A, 94, ASN, O 3.33Å
B, 98, LEU, HA A, 97, GLY, O 2.52Å
B, 101, GLY, HA1 A, 98, LEU, O 2.89Å
B, 102, LEU, HA A, 101, GLY, O 3.02Å
A, 94, ASN, HA B, 94, ASN, OD1 3.11Å
A, 97, GLY, HA1 B, 94, ASN, OD1 2.40Å
A, 97, GLY, HA1 B, 94, ASN, O 3.33Å
A, 98, LEU, HA B, 97, GLY, O 2.52Å
A, 101, GLY, HA1 B, 98, LEU, O 2.89Å
A, 102, LEU, HA B, 101, GLY, O 3.02Å