Patatin-like phospholipase domain-containing protein 2

UniProt data

UniProt name: PLPL2_HUMAN (Q96AD5)

Gene name: PNPLA2; Synonyms=ATGL; ORFNames=FP17548

Organism: Homo sapiens (Human).

Function: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. {ECO:0000269|PubMed:15364929, ECO:0000269|PubMed:15550674, ECO:0000269|PubMed:16239926}.

Subcellular location: Lipid droplet. Cell membrane; Single-pass type II membrane protein.

Transmembrane domains:

  1. 9-29, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 504 AA

10 20 30 40 50 60 MFPREKTWNI SFAGCGFLGV YYVGVASCLR EHAPFLVANA THIYGASAGA LTATALVTGV 70 80 90 100 110 120 CLGEAGAKFI EVSKEARKRF LGPLHPSFNL VKIIRSFLLK VLPADSHEHA SGRLGISLTR 130 140 150 160 170 180 VSDGENVIIS HFNSKDELIQ ANVCSGFIPV YCGLIPPSLQ GVRYVDGGIS DNLPLYELKN 190 200 210 220 230 240 TITVSPFSGE SDICPQDSST NIHELRVTNT SIQFNLRNLY RLSKALFPPE PLVLREMCKQ 250 260 270 280 290 300 GYRDGLRFLQ RNGLLNRPNP LLALPPARPH GPEDKDQAVE SAQAEDYSQL PGEDHILEHL 310 320 330 340 350 360 PARLNEALLE ACVEPTDLLT TLSNMLPVRL ATAMMVPYTL PLESALSFTI RLLEWLPDVP 370 380 390 400 410 420 EDIRWMKEQT GSICQYLVMR AKRKLGRHLP SRLPEQVELR RVQSLPSVPL SCAAYREALP 430 440 450 460 470 480 GWMRNNLSLG DALAKWEECQ RQLLLGLFCT NVAFPPEALR MRAPADPAPA PADPASPQHQ 490 500 LAGPAPLLST PAPEARPVIG ALGL

CATM prediction

The sequence of PLPL2_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
      10        20        30 
    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 KTWNISFAGCGFLGVYYVGVASCLREH 7.2 -46.7° 2.2 45.1 -5.0
Model 2 KTWNISFAGCGFLGVYYVGVASCLREH 7.3 -48.0° 3.3 34.0 -0.2

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 21, TYR, HA A, 20, VAL, O 3.36Å
B, 24, GLY, HA1 A, 21, TYR, O 3.19Å
A, 21, TYR, HA B, 20, VAL, O 3.36Å
A, 24, GLY, HA1 B, 21, TYR, O 3.19Å