GPI transamidase component PIG-T

UniProt data

UniProt name: PIGT_HUMAN (Q969N2)

Gene name: PIGT; ORFNames=CGI-06, PSEC0163, UNQ716/PRO1379

Organism: Homo sapiens (Human).

Function: Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates. {ECO:0000269|PubMed:11483512}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:11483512}; Single-pass type I membrane protein {ECO:0000269|PubMed:11483512}.

Transmembrane domains:

  1. 528-548, 21 AA, Helical. {ECO:0000255}.

Sequence: 578 AA

10 20 30 40 50 60 MAAAMPLALL VLLLLGPGGW CLAEPPRDSL REELVITPLP SGDVAATFQF RTRWDSELQR 70 80 90 100 110 120 EGVSHYRLFP KALGQLISKY SLRELHLSFT QGFWRTRYWG PPFLQAPSGA ELWVWFQDTV 130 140 150 160 170 180 TDVDKSWKEL SNVLSGIFCA SLNFIDSTNT VTPTASFKPL GLANDTDHYF LRYAVLPREV 190 200 210 220 230 240 VCTENLTPWK KLLPCSSKAG LSVLLKADRL FHTSYHSQAV HIRPVCRNAR CTSISWELRQ 250 260 270 280 290 300 TLSVVFDAFI TGQGKKDWSL FRMFSRTLTE PCPLASESRV YVDITTYNQD NETLEVHPPP 310 320 330 340 350 360 TTTYQDVILG TRKTYAIYDL LDTAMINNSR NLNIQLKWKR PPENEAPPVP FLHAQRYVSG 370 380 390 400 410 420 YGLQKGELST LLYNTHPYRA FPVLLLDTVP WYLRLYVHTL TITSKGKENK PSYIHYQPAQ 430 440 450 460 470 480 DRLQPHLLEM LIQLPANSVT KVSIQFERAL LKWTEYTPDP NHGFYVSPSV LSALVPSMVA 490 500 510 520 530 540 AKPVDWEESP LFNSLFPVSD GSNYFVRLYT EPLLVNLPTP DFSMPYNVIC LTCTVVAVCY 550 560 570 GSFYNLLTRT FHIEEPRTGG LAKRLANLIR RARGVPPL

CATM prediction

The sequence of PIGT_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       530       540       55
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 AYNVICLTCTVVAVCYGSFYNLLTRTF 6.7 -48.8° 1.8 44.9 -32.7
Prolines* PYNVICLTCTVVAVCYGSFYNLLTRTF          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 538, VAL, HA A, 537, ALA, O 3.02Å
B, 541, GLY, HA1 A, 538, VAL, O 2.67Å
B, 542, SER, HA A, 541, GLY, O 3.15Å
B, 545, ASN, HD21 A, 545, ASN, OD1 2.19Å
B, 546, LEU, HA A, 545, ASN, OD1 3.24Å
A, 538, VAL, HA B, 537, ALA, O 3.02Å
A, 541, GLY, HA1 B, 538, VAL, O 2.67Å
A, 542, SER, HA B, 541, GLY, O 3.15Å