GPI-anchor transamidase

UniProt data

UniProt name: GPI8_HUMAN (Q92643)

Gene name: PIGK; Synonyms=GPI8

Organism: Homo sapiens (Human).

Function: Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:11483512}; Single-pass type I membrane protein {ECO:0000269|PubMed:11483512}.

Transmembrane domains:

  1. 368-388, 21 AA, Helical. {ECO:0000255}.

Sequence: 395 AA

10 20 30 40 50 60 MAVTDSLSRA ATVLATVLLL SFGSVAASHI EDQAEQFFRS GHTNNWAVLV CTSRFWFNYR 70 80 90 100 110 120 HVANTLSVYR SVKRLGIPDS HIVLMLADDM ACNPRNPKPA TVFSHKNMEL NVYGDDVEVD 130 140 150 160 170 180 YRSYEVTVEN FLRVLTGRIP PSTPRSKRLL SDDRSNILIY MTGHGGNGFL KFQDSEEITN 190 200 210 220 230 240 IELADAFEQM WQKRRYNELL FIIDTCQGAS MYERFYSPNI MALASSQVGE DSLSHQPDPA 250 260 270 280 290 300 IGVHLMDRYT FYVLEFLEEI NPASQTNMND LFQVCPKSLC VSTPGHRTDL FQRDPKNVLI 310 320 330 340 350 360 TDFFGSVRKV EITTETIKLQ QDSEIMESSY KEDQMDEKLM EPLKYAEQLP VAQIIHQKPK 370 380 390 LKDWHPPGGF ILGLWALIIM VFFKTYGIKH MKFIF

CATM prediction

The sequence of GPI8_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       370       380       39
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 HAAGGFILGLWALIIMVFFKTYGIKHM 7.1 -26.0° 3.6 54.0 -23.0
Model 2 HAAGGFILGLWALIIMVFFKTYGIKHM 6.9 -36.0° 5.1 45.0 -7.6
Prolines* HPPGGFILGLWALIIMVFFKTYGIKHM          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session