Probable E3 ubiquitin-protein ligase RNF217

UniProt data

UniProt name: RN217_HUMAN (Q8TC41)

Gene name: RNF217; Synonyms=C6orf172, IBRDC1

Organism: Homo sapiens (Human).

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. {ECO:0000250}.

Subcellular location: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 503-523, 21 AA, Helical. {ECO:0000255}.

Sequence: 542 AA

10 20 30 40 50 60 MGEEQSTVSG GGGPQESQTL ASGTAGHPEP PRPQGDSARA PPLRAASAEP SGGGCGSDWG 70 80 90 100 110 120 CADTSAPEPA RSLGPPGWSK SRAPAQPAGL ALTGPLNPQT LPLQLELEEE EEEAGDRKEG 130 140 150 160 170 180 GDEQQEAPPG EELEPRTRVG AADGLVLDVL GQRRPSLAKR QVFCSVYCVE SDLPEAPASE 190 200 210 220 230 240 QLSPPASPPG APPVLNPPST RSSFPSPRLS LPTDSLSPDG GSIELEFYLA PEPFSMPSLL 250 260 270 280 290 300 GAPPYSGLGG VGDPYVPLMV LMCRVCLEDK PIKPLPCCKK AVCEECLKVY LSAQVQLGQV 310 320 330 340 350 360 EIKCPITECF EFLEETTVVY NLTHEDSIKY KYFLELGRID SSTKPCPQCK HFTTFKKKGH 370 380 390 400 410 420 IPTPSRSESK YKIQCPTCQF VWCFKCHSPW HEGVNCKEYK KGDKLLRHWA SEIEHGQRNA 430 440 450 460 470 480 QKCPKCKIHI QRTEGCDHMT CSQCNTNFCY RCGERYRQLR FFGDHTSNLS IFGCKYRYLP 490 500 510 520 530 540 ERPHLRRLVR GSVCAGKLFI APLIMVLGLA LGAIAVVIGL FVFPIYCLCK KQRKRSRTGM HW

CATM prediction

The sequence of RN217_HUMAN returned 4 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
  500       510       520    
|    +    |    +    |    + 		 (Å) (°) (Å) (°)  
Model 1 IAALIMVLGLALGAIAVVIGLFVFAIY 6.8 -47.4° 1.3 45.0 -30.9
Model 2 IAALIMVLGLALGAIAVVIGLFVFAIY 7.0 +54.1° 3.3 80.2 -19.5
Model 3 IAALIMVLGLALGAIAVVIGLFVFAIY 7.2 -50.1° 2.9 47.0 -16.1
Model 4 IAALIMVLGLALGAIAVVIGLFVFAIY 7.3 -54.1° 3.6 49.2 -5.7
Prolines* IAPLIMVLGLALGAIAVVIGLFVFPIY          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 505, MET, HA A, 504, ILE, O 3.18Å
B, 508, GLY, HA1 A, 505, MET, O 2.77Å
B, 509, LEU, HA A, 508, GLY, O 2.95Å
A, 505, MET, HA B, 504, ILE, O 3.20Å
A, 508, GLY, HA1 B, 505, MET, O 2.77Å
A, 509, LEU, HA B, 508, GLY, O 2.95Å