Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

UniProt data

UniProt name: LFNG_HUMAN (Q8NES3)

Gene name: LFNG

Organism: Homo sapiens (Human).

Function: Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning (By similarity). {ECO:0000250}.

Subcellular location: Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.

Transmembrane domains:

  1. 9-29, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 379 AA

10 20 30 40 50 60 MLKRCGRRLL LALAGALLAC LLVLTADPPP PPLPAERGRR ALRSLAGPAG AAPAPGLGAA 70 80 90 100 110 120 AAAPGALVRD VHSLSEYFSL LTRARRDAGP PPGAAPRPAD GHPRPLAEPL APRDVFIAVK 130 140 150 160 170 180 TTKKFHRARL DLLLETWISR HKEMTFIFTD GEDEALARHT GNVVITNCSA AHSRQALSCK 190 200 210 220 230 240 MAVEYDRFIE SGRKWFCHVD DDNYVNLRAL LRLLASYPHT RDVYVGKPSL DRPIQAMERV 250 260 270 280 290 300 SENKVRPVHF WFATGGAGFC ISRGLALKMS PWASGGHFMN TAERIRLPDD CTIGYIVEAL 310 320 330 340 350 360 LGVPLIRSGL FHSHLENLQQ VPTSELHEQV TLSYGMFENK RNAVHVKGPF SVEADPSRFR 370 SIHCHLYPDT PWCPRTAIF

CATM prediction

The sequence of LFNG_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
      10        20        30 
    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 GRRLLLALAGALLACLLVLTADAAAAA 6.7 -50.8° 1.2 45.0 -10.8
Prolines* GRRLLLALAGALLACLLVLTADPPPPP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 12, ALA, HA A, 11, LEU, O 3.22Å
B, 15, GLY, HA1 A, 12, ALA, O 2.69Å
B, 16, ALA, HA A, 15, GLY, O 3.02Å
A, 12, ALA, HA B, 11, LEU, O 3.22Å
A, 15, GLY, HA1 B, 12, ALA, O 2.69Å
A, 16, ALA, HA B, 15, GLY, O 3.02Å