CATM - Q8N3T1: Polypeptide N-acetylgalactosaminyltransferase 15

UniProt data

Gene name: GALNT15; Synonyms=GALNTL2; ORFNames=UNQ770/PRO1564

Organism: Homo sapiens (Human).

Function: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Although it displays a much weaker activity toward all substrates tested compared to GALNT2, it is able to transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting that it can fill vicinal Thr/Ser residues in cooperation with other GALNT proteins. Prefers Muc1a as substrate.

Subcellular location: Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.

Transmembrane domains:

12-34, 23 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 639 AA

10 20 30 40 50 60 MLLRKRYRHR PCRLQFLLLL LMLGCVLMMV AMLHPPHHTL HQTVTAQASK HSPEARYRLD 70 80 90 100 110 120 FGESQDWVLE AEDEGEEYSP LEGLPPFISL REDQLLVAVA LPQARRNQSQ GRRGGSYRLI 130 140 150 160 170 180 KQPRRQDKEA PKRDWGADED GEVSEEEELT PFSLDPRGLQ EALSARIPLQ RALPEVRHPL 190 200 210 220 230 240 CLQQHPQDSL PTASVILCFH DEAWSTLLRT VHSILDTVPR AFLKEIILVD DLSQQGQLKS 250 260 270 280 290 300 ALSEYVARLE GVKLLRSNKR LGAIRARMLG ATRATGDVLV FMDAHCECHP GWLEPLLSRI 310 320 330 340 350 360 AGDRSRVVSP VIDVIDWKTF QYYPSKDLQR GVLDWKLDFH WEPLPEHVRK ALQSPISPIR 370 380 390 400 410 420 SPVVPGEVVA MDRHYFQNTG AYDSLMSLRG GENLELSFKA WLCGGSVEIL PCSRVGHIYQ 430 440 450 460 470 480 NQDSHSPLDQ EATLRNRVRI AETWLGSFKE TFYKHSPEAF SLSKAEKPDC MERLQLQRRL 490 500 510 520 530 540 GCRTFHWFLA NVYPELYPSE PRPSFSGKLH NTGLGLCADC QAEGDILGCP MVLAPCSDSR 550 560 570 580 590 600 QQQYLQHTSR KEIHFGSPQH LCFAVRQEQV ILQNCTEEGL AIHQQHWDFQ ENGMIVHILS 610 620 630 GKCMEAVVQE NNKDLYLRPC DGKARQQWRF DQINAVDER

CATM prediction

The sequence of GLT15_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

#	Sequence and interface	d	θ	Z'	ω'	Score
	10 20 30 \| + \| + \| +	(Å)	(°)	(Å)	(°)
Model 1	HRACRLQFLLLLLMLGCVLMMVAMLHAAH	6.9	-55.0°	0.9	50.2	-26.2
Prolines*	HRPCRLQFLLLLLMLGCVLMMVAMLHPPH

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:

Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor	Acceptor	Distance
B, 24, GLY, HA1	A, 21, LEU, O	2.97Å
B, 25, CYS, HA	A, 24, GLY, O	3.19Å
A, 24, GLY, HA1	B, 21, LEU, O	2.97Å
A, 25, CYS, HA	B, 24, GLY, O	3.19Å