Ubiquitin-conjugating enzyme E2 J2

UniProt data

UniProt name: UB2J2_HUMAN (Q8N2K1)

Gene name: UBE2J2; Synonyms=NCUBE2

Organism: Homo sapiens (Human).

Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). {ECO:0000255|PROSITE-ProRule:PRU00388}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9Y385}; Single-pass type IV membrane protein {ECO:0000250|UniProtKB:Q9Y385}.

Transmembrane domains:

  1. 227-247, 21 AA, Helical; Anchor for type IV membrane protein. {ECO:0000255}.

Sequence: 259 AA

10 20 30 40 50 60 MSSTSSKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG PEMTPYEGGY 70 80 90 100 110 120 YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF HPDTWNPAWS VSTILTGLLS 130 140 150 160 170 180 FMVEKGPTLG SIETSDFTKR QLAVQSLAFN LKDKVFCELF PEVVEEIKQK QKAQDELSSR 190 200 210 220 230 240 PQTLPLPDVV PDGETHLVQN GIQLLNGHAP GAVPNLAGLQ QANRHHGLLG GALANLFVIV 250 GFAAFAYTVK YVLRSIAQE

CATM prediction

The sequence of UB2J2_HUMAN returned 4 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
        230       240       2
 +    |    +    |    +    |		 (Å) (°) (Å) (°)  
Model 1 RHHGLLGGALANLFVIVGFAAFAYTVK 6.6 -49.3° 1.8 45.0 -29.2
Model 2 RHHGLLGGALANLFVIVGFAAFAYTVK 6.8 -43.2° 3.0 34.8 -16.7
Model 3 RHHGLLGGALANLFVIVGFAAFAYTVK 6.8 -55.0° 4.9 53.3 -15.6
Model 4 RHHGLLGGALANLFVIVGFAAFAYTVK 6.9 -51.8° 1.4 56.5 -4.5

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 228, LEU, HA A, 227, GLY, O 2.95Å
B, 231, GLY, HA1 A, 228, LEU, O 2.56Å
B, 232, ALA, HA A, 231, GLY, O 3.02Å
A, 228, LEU, HA B, 227, GLY, O 2.95Å
A, 231, GLY, HA1 B, 228, LEU, O 2.56Å
A, 232, ALA, HA B, 231, GLY, O 3.02Å
A, 235, ASN, HD21 B, 235, ASN, OD1 1.95Å
A, 236, LEU, HA B, 235, ASN, OD1 3.06Å