Chondroitin sulfate synthase 2

UniProt data

UniProt name: CHSS2_HUMAN (Q8IZ52)

Gene name: CHPF; Synonyms=CSS2; ORFNames=UNQ651/PRO1281

Organism: Homo sapiens (Human).

Function: Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Isoform 2 may facilitate PARK2 transport into the mitochondria. In collaboration with PARK2, isoform 2 may enhance cell viability and protect cells from oxidative stress. {ECO:0000269|PubMed:12761225}.

Subcellular location: Isoform 2: Mitochondrion matrix {ECO:0000269|PubMed:22082830}.

Transmembrane domains:

  1. 16-34, 19 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 775 AA

10 20 30 40 50 60 MRASLLLSVL RPAGPVAVGI SLGFTLSLLS VTWVEEPCGP GPPQPGDSEL PPRGNTNAAR 70 80 90 100 110 120 RPNSVQPGAE REKPGAGEGA GENWEPRVLP YHPAQPGQAA KKAVRTRYIS TELGIRQRLL 130 140 150 160 170 180 VAVLTSQTTL PTLGVAVNRT LGHRLERVVF LTGARGRRAP PGMAVVTLGE ERPIGHLHLA 190 200 210 220 230 240 LRHLLEQHGD DFDWFFLVPD TTYTEAHGLA RLTGHLSLAS AAHLYLGRPQ DFIGGEPTPG 250 260 270 280 290 300 RYCHGGFGVL LSRMLLQQLR PHLEGCRNDI VSARPDEWLG RCILDATGVG CTGDHEGVHY 310 320 330 340 350 360 SHLELSPGEP VQEGDPHFRS ALTAHPVRDP VHMYQLHKAF ARAELERTYQ EIQELQWEIQ 370 380 390 400 410 420 NTSHLAVDGD QAAAWPVGIP APSRPASRFE VLRWDYFTEQ HAFSCADGSP RCPLRGADRA 430 440 450 460 470 480 DVADVLGTAL EELNRRYHPA LRLQKQQLVN GYRRFDPARG MEYTLDLQLE ALTPQGGRRP 490 500 510 520 530 540 LTRRVQLLRP LSRVEILPVP YVTEASRLTV LLPLAAAERD LAPGFLEAFA TAALEPGDAA 550 560 570 580 590 600 AALTLLLLYE PRQAQRVAHA DVFAPVKAHV AELERRFPGA RVPWLSVQTA APSPLRLMDL 610 620 630 640 650 660 LSKKHPLDTL FLLAGPDTVL TPDFLNRCRM HAISGWQAFF PMHFQAFHPA VAPPQGPGPP 670 680 690 700 710 720 ELGRDTGRFD RQAASEACFY NSDYVAARGR LAAASEQEEE LLESLDVYEL FLHFSSLHVL 730 740 750 760 770 RAVEPALLQR YRAQTCSARL SEDLYHRCLQ SVLEGLGSRT QLAMLLFEQE QGNST

CATM prediction

The sequence of CHSS2_HUMAN returned 4 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
         20        30      
  +    |    +    |    +  		 (Å) (°) (Å) (°)  
Model 1 AGAVAVGISLGFTLSLLSVTWVEEA 7.0 -42.1° 1.4 44.7 -31.3
Model 2 AGAVAVGISLGFTLSLLSVTWVEEA 6.9 -43.5° 2.4 44.1 -16.6
Model 3 AGAVAVGISLGFTLSLLSVTWVEEA 7.0 -34.1° 1.7 56.7 -13.3
Model 4 AGAVAVGISLGFTLSLLSVTWVEEA 6.4 -52.8° 5.3 58.0 -9.5
Prolines* AGPVAVGISLGFTLSLLSVTWVEEP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 19, GLY, HA1 A, 16, VAL, O 3.00Å
B, 20, ILE, HA A, 19, GLY, O 2.71Å
A, 19, GLY, HA1 B, 16, VAL, O 3.00Å
A, 20, ILE, HA B, 19, GLY, O 2.71Å
A, 27, SER, HG1 B, 27, SER, OG 2.24Å