Izumo sperm-egg fusion protein 1

UniProt data

UniProt name: IZUM1_HUMAN (Q8IYV9)

Gene name: IZUMO1

Organism: Homo sapiens (Human).

Function: Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. {ECO:0000269|PubMed:15759005}.

Subcellular location: Cell membrane {ECO:0000250|UniProtKB:Q9D9J7}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q9D9J7}.

Transmembrane domains:

  1. 293-313, 21 AA, Helical. {ECO:0000255}.

Sequence: 350 AA

10 20 30 40 50 60 MGPHFTLLCA ALAGCLLPAE GCVICDPSVV LALKSLEKDY LPGHLDAKHH KAMMERVENA 70 80 90 100 110 120 VKDFQELSLN EDAYMGVVDE ATLQKGSWSL LKDLKRITDS DVKGDLFVKE LFWMLHLQKE 130 140 150 160 170 180 TFATYVARFQ KEAYCPNKCG VMLQTLIWCK NCKKEVHACR KSYDCGERNV EVPQMEDMIL 190 200 210 220 230 240 DCELNWHQAS EGLTDYSFYR VWGNNTETLV SKGKEATLTK PMVGPEDAGS YRCELGSVNS 250 260 270 280 290 300 SPATIINFHV TVLPKMIKEE KPSPNIVTPG EATTESSISL QPLQPEKMLA SRLLGLLICG 310 320 330 340 SLALITGLTF AIFRRRKVID FIKSSLFGLG SGAAEQTQVP KEKATDSRQQ

CATM prediction

The sequence of IZUM1_HUMAN returned 4 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
  290       300       310    
|    +    |    +    |    + 		 (Å) (°) (Å) (°)  
Model 1 ASRLLGLLICGSLALITGLTFAIFRRR 7.1 -38.2° 5.7 33.9 -40.0
Model 2 ASRLLGLLICGSLALITGLTFAIFRRR 7.2 -37.0° 2.3 35.5 -13.0
Model 3 ASRLLGLLICGSLALITGLTFAIFRRR 7.3 -38.0° 3.1 35.0 -5.4
Model 4 ASRLLGLLICGSLALITGLTFAIFRRR 7.0 -51.9° 1.3 57.1 -4.9

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 304, LEU, HA A, 303, ALA, O 3.03Å
B, 307, GLY, HA1 A, 304, LEU, O 3.37Å
A, 304, LEU, HA B, 303, ALA, O 3.03Å
A, 307, GLY, HA1 B, 304, LEU, O 3.37Å