Chondroitin sulfate synthase 1

UniProt data

UniProt name: CHSS1_HUMAN (Q86X52)

Gene name: CHSY1; Synonyms=CHSY, CSS1, KIAA0990; ORFNames=UNQ756/PRO1487

Organism: Homo sapiens (Human).

Function: Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Involved in the negative control of osteogenesis likely through the modulation of NOTCH signaling. {ECO:0000269|PubMed:11514575, ECO:0000269|PubMed:21129727}.

Subcellular location: Golgi apparatus, Golgi stack membrane {ECO:0000305|PubMed:11514575}; Single-pass type II membrane protein {ECO:0000305|PubMed:11514575}. Secreted {ECO:0000269|PubMed:21129727}.

Transmembrane domains:

  1. 8-28, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 802 AA

10 20 30 40 50 60 MAARGRRAWL SVLLGLVLGF VLASRLVLPR ASELKRAGPR RRASPEGCRS GQAAASQAGG 70 80 90 100 110 120 ARGDARGAQL WPPGSDPDGG PRDRNFLFVG VMTAQKYLQT RAVAAYRTWS KTIPGKVQFF 130 140 150 160 170 180 SSEGSDTSVP IPVVPLRGVD DSYPPQKKSF MMLKYMHDHY LDKYEWFMRA DDDVYIKGDR 190 200 210 220 230 240 LENFLRSLNS SEPLFLGQTG LGTTEEMGKL ALEPGENFCM GGPGVIMSRE VLRRMVPHIG 250 260 270 280 290 300 KCLREMYTTH EDVEVGRCVR RFAGVQCVWS YEMQQLFYEN YEQNKKGYIR DLHNSKIHQA 310 320 330 340 350 360 ITLHPNKNPP YQYRLHSYML SRKISELRHR TIQLHREIVL MSKYSNTEIH KEDLQLGIPP 370 380 390 400 410 420 SFMRFQPRQR EEILEWEFLT GKYLYSAVDG QPPRRGMDSA QREALDDIVM QVMEMINANA 430 440 450 460 470 480 KTRGRIIDFK EIQYGYRRVN PMYGAEYILD LLLLYKKHKG KKMTVPVRRH AYLQQTFSKI 490 500 510 520 530 540 QFVEHEELDA QELAKRINQE SGSLSFLSNS LKKLVPFQLP GSKSEHKEPK DKKINILIPL 550 560 570 580 590 600 SGRFDMFVRF MGNFEKTCLI PNQNVKLVVL LFNSDSNPDK AKQVELMRDY RIKYPKADMQ 610 620 630 640 650 660 ILPVSGEFSR ALALEVGSSQ FNNESLLFFC DVDLVFTTEF LQRCRANTVL GQQIYFPIIF 670 680 690 700 710 720 SQYDPKIVYS GKVPSDNHFA FTQKTGFWRN YGFGITCIYK GDLVRVGGFD VSIQGWGLED 730 740 750 760 770 780 VDLFNKVVQA GLKTFRSQEV GVVHVHHPVF CDPNLDPKQY KMCLGSKAST YGSTQQLAEM 790 800 WLEKNDPSYS KSSNNNGSVR TA

CATM prediction

The sequence of CHSS1_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       10        20        30
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 GRRAWLSVLLGLVLGFVLASRLVLARA 6.6 -40.7° 5.6 45.2 -15.3
Model 2 GRRAWLSVLLGLVLGFVLASRLVLARA 6.9 -34.1° 1.7 56.7 -15.1
Prolines* GRRAWLSVLLGLVLGFVLASRLVLPRA          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 12, VAL, HA A, 11, SER, O 3.45Å
B, 15, GLY, HA1 A, 12, VAL, O 2.88Å
B, 16, LEU, HA A, 15, GLY, O 2.48Å
B, 19, GLY, HA1 A, 16, LEU, O 3.09Å
A, 12, VAL, HA B, 11, SER, O 3.45Å
A, 15, GLY, HA1 B, 12, VAL, O 2.88Å
A, 16, LEU, HA B, 15, GLY, O 2.48Å
A, 19, GLY, HA1 B, 16, LEU, O 3.09Å