E3 ubiquitin-protein ligase RNF144B

UniProt data

UniProt name: R144B_HUMAN (Q7Z419)

Gene name: RNF144B; Synonyms=IBRDC2, P53RFP

Organism: Homo sapiens (Human).

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se. {ECO:0000269|PubMed:12853982, ECO:0000269|PubMed:20300062}.

Subcellular location: Mitochondrion membrane {ECO:0000269|PubMed:20300062}; Single-pass membrane protein {ECO:0000269|PubMed:20300062}. Cytoplasm {ECO:0000269|PubMed:20300062}. Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.

Transmembrane domains:

  1. 258-278, 21 AA, Helical. {ECO:0000255}.

Sequence: 303 AA

10 20 30 40 50 60 MGSAGRLHYL AMTAENPTPG DLAPAPLITC KLCLCEQSLD KMTTLQECQC IFCTACLKQY 70 80 90 100 110 120 MQLAIREGCG SPITCPDMVC LNHGTLQEAE IACLVPVDQF QLYQRLKFER EVHLDPYRTW 130 140 150 160 170 180 CPVADCQTVC PVASSDPGQP VLVECPSCHL KFCSCCKDAW HAEVSCRDSQ PIVLPTEHRA 190 200 210 220 230 240 LFGTDAEAPI KQCPVCRVYI ERNEGCAQMM CKNCKHTFCW YCLQNLDNDI FLRHYDKGPC 250 260 270 280 290 300 RNKLGHSRAS VMWNRTQVVG ILVGLGIIAL VTSPLLLLAS PCIICCVCKS CRGKKKKHDP STT

CATM prediction

The sequence of R144B_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       260       270       28
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 RTQVVGILVGLGIIALVTSALLLLASA 7.2 -47.0° 1.4 45.0 -12.4
Prolines* RTQVVGILVGLGIIALVTSPLLLLASP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 261, ILE, HA A, 260, GLY, O 3.40Å
A, 261, ILE, HA B, 260, GLY, O 3.40Å