Carbohydrate sulfotransferase 15

UniProt data

UniProt name: CHSTF_HUMAN (Q7LFX5)

Gene name: CHST15; Synonyms=BRAG, GALNAC4S6ST, KIAA0598

Organism: Homo sapiens (Human).

Function: Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. {ECO:0000269|PubMed:11572857, ECO:0000269|PubMed:12874280}.

Subcellular location: Golgi apparatus membrane {ECO:0000305|PubMed:10749872}; Single-pass type II membrane protein {ECO:0000305|PubMed:10749872}. Note=A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.

Transmembrane domains:

  1. 81-101, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 561 AA

10 20 30 40 50 60 MRHCINCCIQ LLPDGAHKQQ VNCQGGPHHG HQACPTCKGE NKILFRVDSK QMNLLAVLEV 70 80 90 100 110 120 RTEGNENWGG FLRFKKGKRC SLVFGLIIMT LVMASYILSG AHQELLISSP FHYGGFPSNP 130 140 150 160 170 180 SLMDSENPSD TKEHHHQSSV NNISYMKDYP SIKLIINSIT TRIEFTTRQL PDLEDLKKQE 190 200 210 220 230 240 LHMFSVIPNK FLPNSKSPCW YEEFSGQNTT DPYLTNSYVL YSKRFRSTFD ALRKAFWGHL 250 260 270 280 290 300 AHAHGKHFRL RCLPHFYIIG QPKCGTTDLY DRLRLHPEVK FSAIKEPHWW TRKRFGIVRL 310 320 330 340 350 360 RDGLRDRYPV EDYLDLFDLA AHQIHQGLQA SSAKEQSKMN TIIIGEASAS TMWDNNAWTF 370 380 390 400 410 420 FYDNSTDGEP PFLTQDFIHA FQPNARLIVM LRDPVERLYS DYLYFASSNK SADDFHEKVT 430 440 450 460 470 480 EALQLFENCM LDYSLRACVY NNTLNNAMPV RLQVGLYAVY LLDWLSVFDK QQFLILRLED 490 500 510 520 530 540 HASNVKYTMH KVFQFLNLGP LSEKQEALMT KSPASNARRP EDRNLGPMWP ITQKILRDFY 550 560 RPFNARLAQV LADEAFAWKT T

CATM prediction

The sequence of CHSTF_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
    80        90        100  
  |    +    |    +    |    		 (Å) (°) (Å) (°)  
Model 1 KRCSLVFGLIIMTLVMASYILSGAHQE 7.3 -45.1° 4.0 39.3 -13.5

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 82, LEU, HA A, 81, SER, O 3.22Å
B, 85, GLY, HA1 A, 82, LEU, O 3.38Å
A, 82, LEU, HA B, 81, SER, O 3.22Å
A, 85, GLY, HA1 B, 82, LEU, O 3.38Å