Cyclic AMP-responsive element-binding protein 3-like protein 2

UniProt data

UniProt name: CR3L2_HUMAN (Q70SY1)

Gene name: CREB3L2; Synonyms=BBF2H7

Organism: Homo sapiens (Human).

Function: Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis by activating the transcription of SEC23A, which promotes the transport and secretion of cartilage matrix proteins, and possibly that of ER biogenesis-related genes (By similarity). In a neuroblastoma cell line, protects cells from ER stress- induced death (PubMed:17178827). In vitro activates transcription of target genes via direct binding to the CRE site (PubMed:17178827). {ECO:0000250|UniProtKB:Q8BH52, ECO:0000269|PubMed:17178827}.

Subcellular location: Processed cyclic AMP-responsive element- binding protein 3-like protein 2: Nucleus. Note=Upon ER stress, translocated into the nucleus. {ECO:0000250|UniProtKB:Q8BH52}.

Transmembrane domains:

  1. 380-400, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 520 AA

10 20 30 40 50 60 MEVLESGEQG VLQWDRKLSE LSEPGDGEAL MYHTHFSELL DEFSQNVLGQ LLNDPFLSEK 70 80 90 100 110 120 SVSMEVEPSP TSPAPLIQAE HSYSLCEEPR AQSPFTHITT SDSFNDDEVE SEKWYLSTDF 130 140 150 160 170 180 PSTSIKTEPV TDEPPPGLVP SVTLTITAIS TPLEKEEPPL EMNTGVDSSC QTIIPKIKLE 190 200 210 220 230 240 PHEVDQFLNF SPKEAPVDHL HLPPTPPSSH GSDSEGSLSP NPRLHPFSLP QTHSPSRAAP 250 260 270 280 290 300 RAPSALSSSP LLTAPHKLQG SGPLVLTEEE KRTLIAEGYP IPTKLPLSKS EEKALKKIRR 310 320 330 340 350 360 KIKNKISAQE SRRKKKEYMD SLEKKVESCS TENLELRKKV EVLENTNRTL LQQLQKLQTL 370 380 390 400 410 420 VMGKVSRTCK LAGTQTGTCL MVVVLCFAVA FGSFFQGYGP YPSATKMALP SQHSLQEPYT 430 440 450 460 470 480 ASVVRSRNLL IYEEHSPPEE SSSPGSAGEL GGWDRGSSLL RVSGLESRPD VDLPHFIISN 490 500 510 ETSLEKSVLL ELQQHLVSAK LEGNETLKVV ELDRRVNTTF

CATM prediction

The sequence of CR3L2_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
     380       390       400 
   |    +    |    +    |   		 (Å) (°) (Å) (°)  
Model 1 GTCLMVVVLCFAVAFGSFFQGYGAYAS 6.9 -48.8° 1.8 45.1 -23.5
Model 2 GTCLMVVVLCFAVAFGSFFQGYGAYAS 7.0 -36.9° 0.0 36.8 -10.1
Prolines* GTCLMVVVLCFAVAFGSFFQGYGPYPS          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 389, VAL, HA A, 388, ALA, O 3.16Å
B, 392, GLY, HA1 A, 389, VAL, O 2.84Å
B, 393, SER, HA A, 392, GLY, O 3.34Å
A, 389, VAL, HA B, 388, ALA, O 3.16Å
A, 392, GLY, HA1 B, 389, VAL, O 2.84Å
A, 393, SER, HA B, 392, GLY, O 3.34Å