Chondroitin sulfate synthase 3

UniProt data

UniProt name: CHSS3_HUMAN (Q70JA7)

Gene name: CHSY3; Synonyms=CHSY2, CSS3

Organism: Homo sapiens (Human).

Function: Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1. {ECO:0000269|PubMed:12907687}.

Subcellular location: Golgi apparatus, Golgi stack membrane {ECO:0000305|PubMed:12907687}; Single-pass type II membrane protein {ECO:0000305|PubMed:12907687}.

Transmembrane domains:

  1. 8-28, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 882 AA

10 20 30 40 50 60 MAVRSRRPWM SVALGLVLGF TAASWLIAPR VAELSERKRR GSSLCSYYGR SAAGPRAGAQ 70 80 90 100 110 120 QPLPQPQSRP RQEQSPPPAR QDLQGPPLPE AAPGITSFRS SPWQQPPPLQ QRRRGREPEG 130 140 150 160 170 180 ATGLPGAPAA EGEPEEEDGG AAGQRRDGRP GSSHNGSGDG GAAAPSARPR DFLYVGVMTA 190 200 210 220 230 240 QKYLGSRALA AQRTWARFIP GRVEFFSSQQ PPNAGQPPPP LPVIALPGVD DSYPPQKKSF 250 260 270 280 290 300 MMIKYMHDHY LDKYEWFMRA DDDVYIKGDK LEEFLRSLNS SKPLYLGQTG LGNIEELGKL 310 320 330 340 350 360 GLEPGENFCM GGPGMIFSRE VLRRMVPHIG ECLREMYTTH EDVEVGRCVR RFGGTQCVWS 370 380 390 400 410 420 YEMQQLFHEN YEHNRKGYIQ DLHNSKIHAA ITLHPNKRPA YQYRLHNYML SRKISELRYR 430 440 450 460 470 480 TIQLHRESAL MSKLSNTEVS KEDQQLGVIP SFNHFQPRER NEVIEWEFLT GKLLYSAAEN 490 500 510 520 530 540 QPPRQSLSSI LRTALDDTVL QVMEMINENA KSRGRLIDFK EIQYGYRRVN PMHGVEYILD 550 560 570 580 590 600 LLLLYKRHKG RKLTVPVRRH AYLQQLFSKP FFRETEELDV NSLVESINSE TQSFSFISNS 610 620 630 640 650 660 LKILSSFQGA KEMGGHNEKK VHILVPLIGR YDIFLRFMEN FENMCLIPKQ NVKLVIILFS 670 680 690 700 710 720 RDSGQDSSKH IELIKGYQNK YPKAEMTLIP MKGEFSRGLG LEMASAQFDN DTLLLFCDVD 730 740 750 760 770 780 LIFREDFLQR CRDNTIQGQQ VYYPIIFSQY DPKVTNGGNP PTDDYFIFSK KTGFWRDYGY 790 800 810 820 830 840 GITCIYKSDL LGAGGFDTSI QGWGLEDVDL YNKVILSGLR PFRSQEVGVV HIFHPVHCDP 850 860 870 880 NLDPKQYKMC LGSKASTFAS TMQLAELWLE KHLGVRYNRT LS

CATM prediction

The sequence of CHSS3_HUMAN returned 4 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       10        20        30
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 SRRAWMSVALGLVLGFTAASWLIAARV 6.9 -38.7° 5.4 59.8 -35.5
Model 2 SRRAWMSVALGLVLGFTAASWLIAARV 6.7 -53.9° 3.0 61.8 -20.6
Model 3 SRRAWMSVALGLVLGFTAASWLIAARV 7.1 -50.9° 3.3 48.0 -12.1
Model 4 SRRAWMSVALGLVLGFTAASWLIAARV 7.3 -44.1° 1.0 45.9 -8.4
Prolines* SRRPWMSVALGLVLGFTAASWLIAPRV          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 12, VAL, HA A, 11, SER, O 3.29Å
B, 15, GLY, HA1 A, 12, VAL, O 3.08Å
B, 16, LEU, HA A, 15, GLY, O 2.89Å
A, 12, VAL, HA B, 11, SER, O 3.29Å
A, 15, GLY, HA1 B, 12, VAL, O 3.08Å
A, 16, LEU, HA B, 15, GLY, O 2.89Å