Ubiquitin carboxyl-terminal hydrolase 30

UniProt data

UniProt name: UBP30_HUMAN (Q70CQ3)

Gene name: USP30 {ECO:0000312|HGNC:HGNC:20065}

Organism: Homo sapiens (Human).

Function: Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PARK2): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951). Preferentially cleaves 'Lys-6'- and 'Lys-11'- linked polyubiquitin chains, 2 types of linkage that participate to mitophagic signaling (PubMed:25621951). Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity). {ECO:0000250|UniProtKB:Q3UN04, ECO:0000269|PubMed:18287522, ECO:0000269|PubMed:24896179, ECO:0000269|PubMed:25527291, ECO:0000269|PubMed:25621951}.

Subcellular location: Mitochondrion outer membrane {ECO:0000269|PubMed:18287522, ECO:0000269|PubMed:24896179}.

Transmembrane domains:

  1. 36-56, 21 AA, Helical. {ECO:0000255}.

Sequence: 517 AA

10 20 30 40 50 60 MLSSRAEAAM TAADRAIQRF LRTGAAVRYK VMKNWGVIGG IAAALAAGIY VIWGPITERK 70 80 90 100 110 120 KRRKGLVPGL VNLGNTCFMN SLLQGLSACP AFIRWLEEFT SQYSRDQKEP PSHQYLSLTL 130 140 150 160 170 180 LHLLKALSCQ EVTDDEVLDA SCLLDVLRMY RWQISSFEEQ DAHELFHVIT SSLEDERDRQ 190 200 210 220 230 240 PRVTHLFDVH SLEQQSEITP KQITCRTRGS PHPTSNHWKS QHPFHGRLTS NMVCKHCEHQ 250 260 270 280 290 300 SPVRFDTFDS LSLSIPAATW GHPLTLDHCL HHFISSESVR DVVCDNCTKI EAKGTLNGEK 310 320 330 340 350 360 VEHQRTTFVK QLKLGKLPQC LCIHLQRLSW SSHGTPLKRH EHVQFNEFLM MDIYKYHLLG 370 380 390 400 410 420 HKPSQHNPKL NKNPGPTLEL QDGPGAPTPV LNQPGAPKTQ IFMNGACSPS LLPTLSAPMP 430 440 450 460 470 480 FPLPVVPDYS SSTYLFRLMA VVVHHGDMHS GHFVTYRRSP PSARNPLSTS NQWLWVSDDT 490 500 510 VRKASLQEVL SSSAYLLFYE RVLSRMQHQS QECKSEE

CATM prediction

The sequence of UBP30_HUMAN returned 5 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
         40        50        
  +    |    +    |    +    		 (Å) (°) (Å) (°)  
Model 1 KNWGVIGGIAAALAAGIYVIWGAITER 6.4 -47.1° 3.3 54.3 -18.1
Model 2 KNWGVIGGIAAALAAGIYVIWGAITER 6.6 -43.0° 2.4 43.9 -16.3
Model 3 KNWGVIGGIAAALAAGIYVIWGAITER 7.0 -38.0° 5.7 33.7 -12.2
Model 4 KNWGVIGGIAAALAAGIYVIWGAITER 7.4 -39.2° 1.1 42.9 -10.3
Model 5 KNWGVIGGIAAALAAGIYVIWGAITER 7.0 -41.9° 1.9 33.1 -6.5
Prolines* KNWGVIGGIAAALAAGIYVIWGPITER          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 37, VAL, HA A, 36, GLY, O 2.44Å
B, 40, GLY, HA1 A, 37, VAL, O 2.58Å
B, 41, ILE, HA A, 40, GLY, O 3.43Å
A, 37, VAL, HA B, 36, GLY, O 2.44Å
A, 40, GLY, HA1 B, 37, VAL, O 2.58Å
A, 41, ILE, HA B, 40, GLY, O 3.43Å