Cytochrome P450 4V2

UniProt data

UniProt name: CP4V2_HUMAN (Q6ZWL3)

Gene name: CYP4V2

Organism: Homo sapiens (Human).

Function: Omega-hydroxylase that oxidizes medium-chain saturated fatty acids and polyunsaturated omega-3 fatty acids, and which plays a role in fatty acid and steroid metabolism in the eye (PubMed:19661213, PubMed:22772592). Catalyzes the omega- hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. The substrate specificity is higher for myristate > laurate > palmitate (C14>C16>C12) (PubMed:19661213). Acts as a polyunsaturated omega-3 fatty acids hydroxylase by mediating oxidation of docosahexaenoate (DHA) to 22-hydroxydocosahexaenoate (PubMed:22772592). Also produces some 21-hydroxydocosahexaenoate. Also converts eicosapentaenoate (EPA) to 20- hydroxyeicosapentaenoate (20-OH-EPA) (PubMed:22772592). {ECO:0000269|PubMed:19661213, ECO:0000269|PubMed:22772592}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:22772592}; Single-pass membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 13-33, 21 AA, Helical. {ECO:0000255}.

Sequence: 525 AA

10 20 30 40 50 60 MAGLWLGLVW QKLLLWGAAS ALSLAGASLV LSLLQRVASY ARKWQQMRPI PTVARAYPLV 70 80 90 100 110 120 GHALLMKPDG REFFQQIIEY TEEYRHMPLL KLWVGPVPMV ALYNAENVEV ILTSSKQIDK 130 140 150 160 170 180 SSMYKFLEPW LGLGLLTSTG NKWRSRRKML TPTFHFTILE DFLDIMNEQA NILVKKLEKH 190 200 210 220 230 240 INQEAFNCFF YITLCALDII CETAMGKNIG AQSNDDSEYV RAVYRMSEMI FRRIKMPWLW 250 260 270 280 290 300 LDLWYLMFKE GWEHKKSLQI LHTFTNSVIA ERANEMNANE DCRGDGRGSA PSKNKRRAFL 310 320 330 340 350 360 DLLLSVTDDE GNRLSHEDIR EEVDTFMFEG HDTTAAAINW SLYLLGSNPE VQKKVDHELD 370 380 390 400 410 420 DVFGKSDRPA TVEDLKKLRY LECVIKETLR LFPSVPLFAR SVSEDCEVAG YRVLKGTEAV 430 440 450 460 470 480 IIPYALHRDP RYFPNPEEFQ PERFFPENAQ GRHPYAYVPF SAGPRNCIGQ KFAVMEEKTI 490 500 510 520 LSCILRHFWI ESNQKREELG LEGQLILRPS NGIWIKLKRR NADER

CATM prediction

The sequence of CP4V2_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
  10        20        30     
|    +    |    +    |    + 		 (Å) (°) (Å) (°)  
Model 1 WQKLLLWGAASALSLAGASLVLSLLQR 7.0 -48.6° 1.8 44.9 -21.6
Model 2 WQKLLLWGAASALSLAGASLVLSLLQR 7.0 -38.2° 5.7 34.1 -11.0

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 14, LEU, HA A, 13, LEU, O 3.32Å
B, 17, GLY, HA1 A, 14, LEU, O 2.97Å
B, 18, ALA, HA A, 17, GLY, O 3.30Å
A, 14, LEU, HA B, 13, LEU, O 3.32Å
A, 17, GLY, HA1 B, 14, LEU, O 2.97Å
A, 18, ALA, HA B, 17, GLY, O 3.30Å