Phospholipase B1, membrane-associated

UniProt data

UniProt name: PLB1_HUMAN (Q6P1J6)

Gene name: PLB1; Synonyms=PLB

Organism: Homo sapiens (Human).

Function: Membrane-associated phospholipase. Exhibits a calcium- independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity). {ECO:0000250}.

Subcellular location: Apical cell membrane {ECO:0000250}; Single- pass type I membrane protein {ECO:0000250}. Note=Present in the intestinal brush border membranes. {ECO:0000250}.

Transmembrane domains:

  1. 1418-1438, 21 AA, Helical. {ECO:0000255}.

Sequence: 1458 AA

10 20 30 40 50 60 MGLRPGIFLL ELLLLLGQGT PQIHTSPRKS TLEGQLWPET LKNSPFPCNP NKLGVNMPSK 70 80 90 100 110 120 SVHSLKPSDI KFVAAIGNLE IPPDPGTGDL EKQDWTERPQ QVCMGVMTVL SDIIRYFSPS 130 140 150 160 170 180 VPMPVCHTGK RVIPHDGAED LWIQAQELVR NMKENLQLDF QFDWKLINVF FSNASQCYLC 190 200 210 220 230 240 PSAQQNGLAA GGVDELMGVL DYLQQEVPRA FVNLVDLSEV AEVSRQYHGT WLSPAPEPCN 250 260 270 280 290 300 CSEETTRLAK VVMQWSYQEA WNSLLASSRY SEQESFTVVF QPFFYETTPS LHSEDPRLQD 310 320 330 340 350 360 STTLAWHLWN RMMEPAGEKD EPLSVKHGRP MKCPSQESPY LFSYRNSNYL TRLQKPQDKL 370 380 390 400 410 420 EVREGAEIRC PDKDPSDTVP TSVHRLKPAD INVIGALGDS LTAGNGAGST PGNVLDVLTQ 430 440 450 460 470 480 YRGLSWSVGG DENIGTVTTL ANILREFNPS LKGFSVGTGK ETSPNAFLNQ AVAGGRAEDL 490 500 510 520 530 540 PVQARRLVDL MKNDTRIHFQ EDWKIITLFI GGNDLCDFCN DLVHYSPQNF TDNIGKALDI 550 560 570 580 590 600 LHAEVPRAFV NLVTVLEIVN LRELYQEKKV YCPRMILRSL CPCVLKFDDN STELATLIEF 610 620 630 640 650 660 NKKFQEKTHQ LIESGRYDTR EDFTVVVQPF FENVDMPKTS EGLPDNSFFA PDCFHFSSKS 670 680 690 700 710 720 HSRAASALWN NMLEPVGQKT TRHKFENKIN ITCPNQVQPF LRTYKNSMQG HGTWLPCRDR 730 740 750 760 770 780 APSALHPTSV HALRPADIQV VAALGDSLTA GNGIGSKPDD LPDVTTQYRG LSYSAGGDGS 790 800 810 820 830 840 LENVTTLPNI LREFNRNLTG YAVGTGDAND TNAFLNQAVP GAKAEDLMSQ VQTLMQKMKD 850 860 870 880 890 900 DHRVNFHEDW KVITVLIGGS DLCDYCTDSN LYSAANFVHH LRNALDVLHR EVPRVLVNLV 910 920 930 940 950 960 DFLNPTIMRQ VFLGNPDKCP VQQASVLCNC VLTLRENSQE LARLEAFSRA YRSSMRELVG 970 980 990 1000 1010 1020 SGRYDTQEDF SVVLQPFFQN IQLPVLADGL PDTSFFAPDC IHPNQKFHSQ LARALWTNML 1030 1040 1050 1060 1070 1080 EPLGSKTETL DLRAEMPITC PTQNEPFLRT PRNSNYTYPI KPAIENWGSD FLCTEWKASN 1090 1100 1110 1120 1130 1140 SVPTSVHQLR PADIKVVAAL GDSLTTAVGA RPNNSSDLPT SWRGLSWSIG GDGNLETHTT 1150 1160 1170 1180 1190 1200 LPNILKKFNP YLLGFSTSTW EGTAGLNVAA EGARARDMPA QAWDLVERMK NSPDINLEKD 1210 1220 1230 1240 1250 1260 WKLVTLFIGV NDLCHYCENP EAHLATEYVQ HIQQALDILS EELPRAFVNV VEVMELASLY 1270 1280 1290 1300 1310 1320 QGQGGKCAML AAQNNCTCLR HSQSSLEKQE LKKVNWNLQH GISSFSYWHQ YTQREDFAVV 1330 1340 1350 1360 1370 1380 VQPFFQNTLT PLNERGDTDL TFFSEDCFHF SDRGHAEMAI ALWNNMLEPV GRKTTSNNFT 1390 1400 1410 1420 1430 1440 HSRAKLKCPS PESPYLYTLR NSRLLPDQAE EAPEVLYWAV PVAAGVGLVV GIIGTVVWRC 1450 RRGGRREDPP MSLRTVAL

CATM prediction

The sequence of PLB1_HUMAN returned 5 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       1420      1430      14
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 VLYWAVAVAAGVGLVVGIIGTVVWRCR 7.0 -42.2° 1.3 60.3 -26.4
Model 2 VLYWAVAVAAGVGLVVGIIGTVVWRCR 6.6 -43.2° 6.0 44.0 -21.0
Model 3 VLYWAVAVAAGVGLVVGIIGTVVWRCR 6.6 +54.1° 3.3 80.2 -14.6
Model 4 VLYWAVAVAAGVGLVVGIIGTVVWRCR 7.3 -47.1° 2.3 44.8 -9.3
Model 5 VLYWAVAVAAGVGLVVGIIGTVVWRCR 7.2 -43.6° 5.0 43.9 -2.9
Prolines* VLYWAVPVAAGVGLVVGIIGTVVWRCR          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 1424, ALA, HA A, 1423, ALA, O 2.69Å
B, 1427, GLY, HA1 A, 1424, ALA, O 2.78Å
B, 1428, LEU, HA A, 1427, GLY, O 3.29Å
A, 1424, ALA, HA B, 1423, ALA, O 2.69Å
A, 1427, GLY, HA1 B, 1424, ALA, O 2.78Å
A, 1428, LEU, HA B, 1427, GLY, O 3.29Å