Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B

UniProt data

UniProt name: MGT5B_HUMAN (Q3V5L5)

Gene name: MGAT5B; Synonyms=KIAA2008

Organism: Homo sapiens (Human).

Function: Glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans. Catalyzes the transfer of N- acetylglucosamine (GlcNAc) to the beta 1-6 linkage of the mannose residue of GlcNAcbeta1,2-Manalpha on both the alpha1,3- and alpha1,6-linked mannose arms in the core structure of N-glycan. Also acts on the GlcNAcbeta1,2-Manalpha1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan. Plays an active role in modulating integrin and laminin-dependent adhesion and migration of neuronal cells via its activity in the O-mannosyl glycan pathway. {ECO:0000269|PubMed:12941944, ECO:0000269|PubMed:14617637, ECO:0000269|PubMed:14623122, ECO:0000269|PubMed:16606368, ECO:0000269|PubMed:16857188}.

Subcellular location: Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.

Transmembrane domains:

  1. 25-45, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 792 AA

10 20 30 40 50 60 MITVNPDGKI MVRRCLVTLR PFRLFVLGIG FFTLCFLMTS LGGQFSARRL GDSPFTIRTE 70 80 90 100 110 120 VMGGPESRGV LRKMSDLLEL MVKRMDALAR LENSSELHRA GGDLHFPADR MPPGAGLMER 130 140 150 160 170 180 IQAIAQNVSD IAVKVDQILR HSLLLHSKVS EGRRDQCEAP SDPKFPDCSG KVEWMRARWT 190 200 210 220 230 240 SDPCYAFFGV DGTECSFLIY LSEVEWFCPP LPWRNQTAAQ RAPKPLPKVQ AVFRSNLSHL 250 260 270 280 290 300 LDLMGSGKES LIFMKKRTKR LTAQWALAAQ RLAQKLGATQ RDQKQILVHI GFLTEESGDV 310 320 330 340 350 360 FSPRVLKGGP LGEMVQWADI LTALYVLGHG LRVTVSLKEL QSNLGVPPGR GSCPLTMPLP 370 380 390 400 410 420 FDLIYTDYHG LQQMKRHMGL SFKKYRCRIR VIDTFGTEPA YNHEEYATLH GYRTNWGYWN 430 440 450 460 470 480 LNPKQFMTMF PHTPDNSFMG FVSEELNETE KRLIKGGKAS NMAVVYGKEA SIWKLQGKEK 490 500 510 520 530 540 FLGILNKYME IHGTVYYESQ RPPEVPAFVK NHGLLPQPEF QQLLRKAKLF IGFGFPYEGP 550 560 570 580 590 600 APLEAIANGC IFLQSRFSPP HSSLNHEFFR GKPTSREVFS QHPYAENFIG KPHVWTVDYN 610 620 630 640 650 660 NSEEFEAAIK AIMRTQVDPY LPYEYTCEGM LERIHAYIQH QDFCRAPDPA LPEAHAPQSP 670 680 690 700 710 720 FVLAPNATHL EWARNTSLAP GAWPPAHALR AWLAVPGRAC TDTCLDHGLI CEPSFFPFLN 730 740 750 760 770 780 SQDAFLKLQV PCDSTESEMN HLYPAFAQPG QECYLQKEPL LFSCAGSNTK YRRLCPCRDF 790 RKGQVALCQG CL

CATM prediction

The sequence of MGT5B_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
          30        40       
   +    |    +    |    +   		 (Å) (°) (Å) (°)  
Model 1 FRLFVLGIGFFTLCFLMTSLGGQFSAR 6.5 -49.0° 1.8 44.9 -20.7

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 39, THR, HA A, 38, MET, O 2.82Å
B, 42, GLY, HA1 A, 39, THR, O 2.45Å
B, 43, GLY, HA2 A, 42, GLY, O 2.95Å
A, 39, THR, HA B, 38, MET, O 2.82Å
A, 42, GLY, HA1 B, 39, THR, O 2.45Å
A, 43, GLY, HA2 B, 42, GLY, O 2.95Å