Prostasin

UniProt data

UniProt name: PRSS8_HUMAN (Q16651)

Gene name: PRSS8

Organism: Homo sapiens (Human).

Function: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). {ECO:0000269|PubMed:15246975, ECO:0000269|PubMed:15474520}.

Subcellular location: Prostasin heavy chain: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus.

Transmembrane domains:

  1. 320-340, 21 AA, Helical. {ECO:0000255}.

Sequence: 343 AA

10 20 30 40 50 60 MAQKGVLGPG QLGAVAILLY LGLLRSGTGA EGAEAPCGVA PQARITGGSS AVAGQWPWQV 70 80 90 100 110 120 SITYEGVHVC GGSLVSEQWV LSAAHCFPSE HHKEAYEVKL GAHQLDSYSE DAKVSTLKDI 130 140 150 160 170 180 IPHPSYLQEG SQGDIALLQL SRPITFSRYI RPICLPAANA SFPNGLHCTV TGWGHVAPSV 190 200 210 220 230 240 SLLTPKPLQQ LEVPLISRET CNCLYNIDAK PEEPHFVQED MVCAGYVEGG KDACQGDSGG 250 260 270 280 290 300 PLSCPVEGLW YLTGIVSWGD ACGARNRPGV YTLASSYASW IQSKVTELQP RVVPQTQESQ 310 320 330 340 PDSNLCGSHL AFSSAPAQGL LRPILFLPLG LALGLLSPWL SEH

CATM prediction

The sequence of PRSS8_HUMAN returned 6 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
     320       330       340 
   |    +    |    +    |   		 (Å) (°) (Å) (°)  
Model 1 AQGLLRAILFLALGLALGLLSAWLSEH 6.3 -39.0° 1.1 42.8 -57.8
Model 2 AQGLLRAILFLALGLALGLLSAWLSEH 7.3 -19.8° 2.1 44.9 -40.6
Model 3 AQGLLRAILFLALGLALGLLSAWLSEH 7.3 -25.8° 4.5 37.2 -40.0
Model 4 AQGLLRAILFLALGLALGLLSAWLSEH 7.0 -48.7° 2.3 58.9 -30.4
Model 5 AQGLLRAILFLALGLALGLLSAWLSEH 7.2 -55.1° 2.4 47.9 -25.0
Model 6 AQGLLRAILFLALGLALGLLSAWLSEH 7.2 -37.0° 2.2 36.1 -8.2
Prolines* AQGLLRPILFLPLGLALGLLSPWLSEH          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 327, LEU, HA A, 326, PHE, O 2.68Å
B, 330, GLY, HA1 A, 327, LEU, O 2.39Å
B, 331, LEU, HA A, 330, GLY, O 2.40Å
B, 334, GLY, HA1 A, 331, LEU, O 3.24Å
A, 327, LEU, HA B, 326, PHE, O 2.68Å
A, 330, GLY, HA1 B, 327, LEU, O 2.39Å
A, 331, LEU, HA B, 330, GLY, O 2.40Å
A, 334, GLY, HA1 B, 331, LEU, O 3.24Å