Exostosin-1

UniProt data

UniProt name: EXT1_HUMAN (Q16394)

Gene name: EXT1

Organism: Homo sapiens (Human).

Function: Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000269|PubMed:11518722, ECO:0000269|PubMed:22660413}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:10679296}; Single-pass type II membrane protein {ECO:0000269|PubMed:10679296}. Golgi apparatus membrane {ECO:0000269|PubMed:10679296}; Single-pass type II membrane protein {ECO:0000269|PubMed:10679296}. Note=The EXT1/EXT2 complex is localized in the Golgi apparatus.

Transmembrane domains:

  1. 8-28, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 746 AA

10 20 30 40 50 60 MQAKKRYFIL LSAGSCLALL FYFGGLQFRA SRSHSRREEH SGRNGLHHPS PDHFWPRFPD 70 80 90 100 110 120 ALRPFVPWDQ LENEDSSVHI SPRQKRDANS SIYKGKKCRM ESCFDFTLCK KNGFKVYVYP 130 140 150 160 170 180 QQKGEKIAES YQNILAAIEG SRFYTSDPSQ ACLFVLSLDT LDRDQLSPQY VHNLRSKVQS 190 200 210 220 230 240 LHLWNNGRNH LIFNLYSGTW PDYTEDVGFD IGQAMLAKAS ISTENFRPNF DVSIPLFSKD 250 260 270 280 290 300 HPRTGGERGF LKFNTIPPLR KYMLVFKGKR YLTGIGSDTR NALYHVHNGE DVVLLTTCKH 310 320 330 340 350 360 GKDWQKHKDS RCDRDNTEYE KYDYREMLHN ATFCLVPRGR RLGSFRFLEA LQAACVPVML 370 380 390 400 410 420 SNGWELPFSE VINWNQAAVI GDERLLLQIP STIRSIHQDK ILALRQQTQF LWEAYFSSVE 430 440 450 460 470 480 KIVLTTLEII QDRIFKHISR NSLIWNKHPG GLFVLPQYSS YLGDFPYYYA NLGLKPPSKF 490 500 510 520 530 540 TAVIHAVTPL VSQSQPVLKL LVAAAKSQYC AQIIVLWNCD KPLPAKHRWP ATAVPVVVIE 550 560 570 580 590 600 GESKVMSSRF LPYDNIITDA VLSLDEDTVL STTEVDFAFT VWQSFPERIV GYPARSHFWD 610 620 630 640 650 660 NSKERWGYTS KWTNDYSMVL TGAAIYHKYY HYLYSHYLPA SLKNMVDQLA NCEDILMNFL 670 680 690 700 710 720 VSAVTKLPPI KVTQKKQYKE TMMGQTSRAS RWADPDHFAQ RQSCMNTFAS WFGYMPLIHS 730 740 QMRLDPVLFK DQVSILRKKY RDIERL

CATM prediction

The sequence of EXT1_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       10        20        30
+    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 KRYFILLSAGSCLALLFYFGGLQFRAS 6.9 -47.0° 1.3 45.1 -12.4
Model 2 KRYFILLSAGSCLALLFYFGGLQFRAS 7.1 -55.1° 2.4 47.9 -5.4

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 11, LEU, HA A, 10, LEU, O 3.28Å
B, 14, GLY, HA1 A, 11, LEU, O 2.91Å
B, 15, SER, HA A, 14, GLY, O 3.20Å
A, 11, LEU, HA B, 10, LEU, O 3.28Å
A, 14, GLY, HA1 B, 11, LEU, O 2.91Å
A, 15, SER, HA B, 14, GLY, O 3.20Å