Semaphorin-5A

UniProt data

UniProt name: SEM5A_HUMAN (Q13591)

Gene name: SEMA5A; Synonyms=SEMAF

Organism: Homo sapiens (Human).

Function: Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. {ECO:0000250, ECO:0000269|PubMed:15218527, ECO:0000269|PubMed:19850054, ECO:0000269|PubMed:20696765, ECO:0000269|PubMed:21706053}.

Subcellular location: Membrane; Single-pass type I membrane protein.

Transmembrane domains:

  1. 969-989, 21 AA, Helical. {ECO:0000255}.

Sequence: 1074 AA

10 20 30 40 50 60 MKGTCVIAWL FSSLGLWRLA HPEAQGTTQC QRTEHPVISY KEIGPWLREF RAKNAVDFSQ 70 80 90 100 110 120 LTFDPGQKEL VVGARNYLFR LQLEDLSLIQ AVEWECDEAT KKACYSKGKS KEECQNYIRV 130 140 150 160 170 180 LLVGGDRLFT CGTNAFTPVC TNRSLSNLTE IHDQISGMAR CPYSPQHNST ALLTAGGELY 190 200 210 220 230 240 AATAMDFPGR DPAIYRSLGI LPPLRTAQYN SKWLNEPNFV SSYDIGNFTY FFFRENAVEH 250 260 270 280 290 300 DCGKTVFSRA ARVCKNDIGG RFLLEDTWTT FMKARLNCSR PGEVPFYYNE LQSTFFLPEL 310 320 330 340 350 360 DLIYGIFTTN VNSIAASAVC VFNLSAIAQA FSGPFKYQEN SRSAWLPYPN PNPHFQCGTV 370 380 390 400 410 420 DQGLYVNLTE RNLQDAQKFI LMHEVVQPVT TVPSFMEDNS RFSHVAVDVV QGREALVHII 430 440 450 460 470 480 YLATDYGTIK KVRVPLNQTS SSCLLEEIEL FPERRREPIR SLQILHSQSV LFVGLREHVV 490 500 510 520 530 540 KIPLKRCQFY RTRSTCIGAQ DPYCGWDVVM KKCTSLEESL SMTQWEQSIS ACPTRNLTVD 550 560 570 580 590 600 GHFGVWSPWT PCTHTDGSAV GSCLCRTRSC DSPAPQCGGW QCEGPGMEIA NCSRNGGWTP 610 620 630 640 650 660 WTSWSPCSTT CGIGFQVRQR SCSNPTPRHG GRVCVGQNRE ERYCNEHLLC PPHMFWTGWG 670 680 690 700 710 720 PWERCTAQCG GGIQARRRIC ENGPDCAGCN VEYQSCNTNP CPELKKTTPW TPWTPVNISD 730 740 750 760 770 780 NGGHYEQRFR YTCKARLADP NLLEVGRQRI EMRYCSSDGT SGCSTDGLSG DFLRAGRYSA 790 800 810 820 830 840 HTVNGAWSAW TSWSQCSRDC SRGIRNRKRV CNNPEPKYGG MPCLGPSLEY QECNILPCPV 850 860 870 880 890 900 DGVWSCWSPW TKCSATCGGG HYMRTRSCSN PAPAYGGDIC LGLHTEEALC NTQPCPESWS 910 920 930 940 950 960 EWSDWSECEA SGVQVRARQC ILLFPMGSQC SGNTTESRPC VFDSNFIPEV SVARSSSVEE 970 980 990 1000 1010 1020 KRCGEFNMFH MIAVGLSSSI LGCLLTLLVY TYCQRYQQQS HDATVIHPVS PAPLNTSITN 1030 1040 1050 1060 1070 HINKLDKYDS VEAIKAFNKN NLILEERNKY FNPHLTGKTY SNAYFTDLNN YDEY

CATM prediction

The sequence of SEM5A_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
      970       980       990
    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 FNMFHMIAVGLSSSILGCLLTLLVYTY 7.1 -43.0° 4.5 35.0 -36.3
Model 2 FNMFHMIAVGLSSSILGCLLTLLVYTY 7.3 -49.6° 2.9 47.0 -17.8
Model 3 FNMFHMIAVGLSSSILGCLLTLLVYTY 7.1 -40.0° 2.7 33.9 -12.3

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 979, SER, HA A, 978, SER, O 3.16Å
B, 982, GLY, HA1 A, 979, SER, O 3.29Å
B, 983, CYS, HA A, 986, THR, OG1 2.97Å
A, 975, GLY, HA2 B, 978, SER, OG 2.79Å
A, 979, SER, HA B, 978, SER, O 3.16Å
A, 982, GLY, HA1 B, 979, SER, O 3.29Å
A, 983, CYS, HA B, 986, THR, OG1 2.97Å