Alpha-2,8-sialyltransferase 8F

UniProt data

UniProt name: SIA8F_HUMAN (P61647)

Gene name: ST8SIA6; Synonyms=SIAT8F

Organism: Homo sapiens (Human).

Function: Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc- alpha-2,3(6)-Gal sequence at the non-reducing end of their carbohydrate groups (By similarity). {ECO:0000250}.

Subcellular location: Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 4-24, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 398 AA

10 20 30 40 50 60 MRPGGALLAL LASLLLLLLL RLLWCPADAP GRARILVEES REATHGTPAA LRTLRSPATA 70 80 90 100 110 120 VPRATNSTYL NEKSLQLTEK CKNLQYGIES FSNKTKGYSE NDYLQIITDI QSCPWKRQAE 130 140 150 160 170 180 EYANFRAKLA SCCDAVQNFV VSQNNTPVGT NMSYEVESKK EIPIKKNIFH MFPVSQPFVD 190 200 210 220 230 240 YPYNQCAVVG NGGILNKSLC GTEIDKSDFV FRCNLPPTTG DVSKDVGSKT NLVTINPSII 250 260 270 280 290 300 TLKYGNLKEK KALFLEDIAT YGDAFFLLPA FSFRANTGTS FKVYYTLEES KARQKVLFFH 310 320 330 340 350 360 PKYLKDLALF WRTKGVTAYR LSTGLMITSV AVELCKNVKL YGFWPFSKTV EDIPVSHHYY 370 380 390 DNKLPKHGFH QMPKEYSQIL QLHMKGILKL QFSKCEVA

CATM prediction

The sequence of SIA8F_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
           10        20      
    +    |    +    |    +  		 (Å) (°) (Å) (°)  
Model 1 MRAGGALLALLASLLLLLLLRLLWCAA 7.3 -36.0° 1.5 36.0 -12.2
Model 2 MRAGGALLALLASLLLLLLLRLLWCAA 6.5 -49.0° 1.8 45.0 -7.8
Prolines* MRPGGALLALLASLLLLLLLRLLWCPA          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 6, ALA, HA A, 5, GLY, O 3.35Å
A, 6, ALA, HA B, 5, GLY, O 3.35Å