Zona pellucida sperm-binding protein 1

UniProt data

UniProt name: ZP1_HUMAN (P60852)

Gene name: ZP1

Organism: Homo sapiens (Human).

Function: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.

Subcellular location: Cell membrane {ECO:0000250|UniProtKB:P48829}; Single-pass type I membrane protein {ECO:0000255}.

Transmembrane domains:

  1. 602-622, 21 AA, Helical. {ECO:0000255}.

Sequence: 638 AA

10 20 30 40 50 60 MAGGSATTWG YPVALLLLVA TLGLGRWLQP DPGLPGLRHS YDCGIKGMQL LVFPRPGQTL 70 80 90 100 110 120 RFKVVDEFGN RFDVNNCSIC YHWVTSRPQE PAVFSADYRG CHVLEKDGRF HLRVFMEAVL 130 140 150 160 170 180 PNGRVDVAQD ATLICPKPDP SRTLDSQLAP PAMFSVSTPQ TLSFLPTSGH TSQGSGHAFP 190 200 210 220 230 240 SPLDPGHSSV HPTPALPSPG PGPTLATLAQ PHWGTLEHWD VNKRDYIGTH LSQEQCQVAS 250 260 270 280 290 300 GHLPCIVRRT SKEACQQAGC CYDNTREVPC YYGNTATVQC FRDGYFVLVV SQEMALTHRI 310 320 330 340 350 360 TLANIHLAYA PTSCSPTQHT EAFVVFYFPL THCGTTMQVA GDQLIYENWL VSGIHIQKGP 370 380 390 400 410 420 QGSITRDSTF QLHVRCVFNA SDFLPIQASI FPPPSPAPMT QPGPLRLELR IAKDETFSSY 430 440 450 460 470 480 YGEDDYPIVR LLREPVHVEV RLLQRTDPNL VLLLHQCWGA PSANPFQQPQ WPILSDGCPF 490 500 510 520 530 540 KGDSYRTQMV ALDGATPFQS HYQRFTVATF ALLDSGSQRA LRGLVYLFCS TSACHTSGLE 550 560 570 580 590 600 TCSTACSTGT TRQRRSSGHR NDTARPQDIV SSPGPVGFED SYGQEPTLGP TDSNGNSSLR 610 620 630 PLLWAVLLLP AVALVLGFGV FVGLSQTWAQ KLWESNRQ

CATM prediction

The sequence of ZP1_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
   600       610       620   
 |    +    |    +    |    +		 (Å) (°) (Å) (°)  
Model 1 LRALLWAVLLLAAVALVLGFGVFVGLS 6.9 -41.0° 0.0 58.9 -33.5
Model 2 LRALLWAVLLLAAVALVLGFGVFVGLS 7.3 -55.2° 0.3 45.0 -16.0
Prolines* LRPLLWAVLLLPAVALVLGFGVFVGLS          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 616, LEU, HA A, 615, VAL, O 3.42Å
B, 619, GLY, HA1 A, 616, LEU, O 3.20Å
B, 620, VAL, HA A, 619, GLY, O 2.99Å
A, 616, LEU, HA B, 615, VAL, O 3.42Å
A, 619, GLY, HA1 B, 616, LEU, O 3.20Å
A, 620, VAL, HA B, 619, GLY, O 2.99Å