Ellis-van Creveld syndrome protein

UniProt data

UniProt name: EVC_HUMAN (P57679)

Gene name: EVC

Organism: Homo sapiens (Human).

Function: Acts as a positive mediator of Hedgehog signaling indispensable for normal endochondral growth and skeletal development. {ECO:0000250}.

Subcellular location: Cell membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250}. Cell projection, cilium {ECO:0000250}. Cell projection, cilium membrane {ECO:0000250}. Note=EVC2 is required for the localization of EVC at the base of primary cilia. {ECO:0000250}.

Transmembrane domains:

  1. 26-48, 23 AA, Helical. {ECO:0000255}.

Sequence: 992 AA

10 20 30 40 50 60 MARGGAACKS DARLLLGRDA LRPAPALLAP AVLLGAALGL GLGLWLGCRA GRQRTRHQKD 70 80 90 100 110 120 DTQNLLKNLE SNAQTPSETG SPSRRRKREV QMSKDKEAVD ECEPPSNSNI TAFALKAKVI 130 140 150 160 170 180 YPINQKFRPL ADGSSNPSLH ENLKQAVLPH QPVEASPSSS LGSLSQGEKD DCSSSSSVHS 190 200 210 220 230 240 ATSDDRFLSR TFLRVNAFPE VLACESVDVD LCIYSLHLKD LLHLDTALRQ EKHMMFIQIF 250 260 270 280 290 300 KMCLLDLLPK KKSDDELYQK ILSKQEKDLE ELEKGLQVKL SNTEMSGAGD SEYITLADVE 310 320 330 340 350 360 KKEREYSEQL IDNMEAFWKQ MANIQHFLVD QFKCSSSKAR QLMMTLTERM IAAEGLLCDS 370 380 390 400 410 420 QELQALDALE RTMGRAHMAK VIEFLKLQVQ EETRCRLAAI SHGLELLAGE GKLSGRQKEE 430 440 450 460 470 480 LLTQQHKAFW QEAERFSREF VQRGKDLVTA SLAHQVEGTA KLTLAQEEEQ RSFLAEAQPT 490 500 510 520 530 540 ADPEKFLEAF HEVLERQRLM QCDLEEEENV RATEAVVALC QELYFSTVDT FQKFVDALFL 550 560 570 580 590 600 QTLPGMTGLP PEECDYLRQE VQENAAWQLG KSNRFRRQQW KLFQELLEQD QQVWMEECAL 610 620 630 640 650 660 SSVLQTHLRE DHEGTIRGVL GRLGGLTEES TRCVLQGHDL LLRSALRRLA LRGNALATLT 670 680 690 700 710 720 QMRLSGKKHL LQELREQRAL EQGSSQCLDE HQWQLLRALE ARVLEEASRL EEEAQQTRLQ 730 740 750 760 770 780 LQQRLLAEAQ EVGQLLQQHM ECAIGQALLV HARNAATKSR AKDRDDFKRT LMEAAVESVY 790 800 810 820 830 840 VTSAGVSRLV QAYYQQIGRI MEDHEERKLQ HLKTLQGERM ENYKLRKKQE LSNPSSGSRT 850 860 870 880 890 900 AGGAHETSQA VHQRMLSQQK RFLAQFPVHQ QMRLHAQQQQ AGVMDLLEAQ LETQLQEAEQ 910 920 930 940 950 960 NFISELAALA RVPLAESKLL PAKRGLLEKP LRTKRKKPLP QERGDLGVPN NEDLASGDQT 970 980 990 SGSLSSKRLS QQESEAGDSG NSKKMLKRRS NL

CATM prediction

The sequence of EVC_HUMAN returned 12 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
         30        40        50
  +    |    +    |    +    | 		 (Å) (°) (Å) (°)  
Model 1 AAAALLAAAVLLGAALGLGLGLWLGCRAG 7.0 -37.1° -0.0 37.3 -41.3
Model 2 AAAALLAAAVLLGAALGLGLGLWLGCRAG 6.7 -33.8° 4.1 62.2 -39.3
Model 3 AAAALLAAAVLLGAALGLGLGLWLGCRAG 6.5 -33.1° -0.0 45.1 -36.8
Model 4 AAAALLAAAVLLGAALGLGLGLWLGCRAG 6.4 -35.7° 3.0 46.0 -36.5
Model 5 AAAALLAAAVLLGAALGLGLGLWLGCRAG 6.3 -45.1° 4.3 54.9 -29.9
Model 6 AAAALLAAAVLLGAALGLGLGLWLGCRAG 6.5 -47.8° 0.8 53.6 -20.8
Model 7 AAAALLAAAVLLGAALGLGLGLWLGCRAG 7.0 -54.0° 4.8 41.5 -18.0
Model 8 AAAALLAAAVLLGAALGLGLGLWLGCRAG 6.4 -44.8° 4.3 55.0 -15.3
Model 9 AAAALLAAAVLLGAALGLGLGLWLGCRAG 7.2 -40.1° 2.1 56.2 -9.9
Model 10 AAAALLAAAVLLGAALGLGLGLWLGCRAG 7.1 -53.9° 2.5 33.2 -4.8
Model 11 AAAALLAAAVLLGAALGLGLGLWLGCRAG 7.3 -54.0° 3.6 49.1 -4.2
Model 12 AAAALLAAAVLLGAALGLGLGLWLGCRAG 7.4 -54.0° 2.9 50.9 -2.9
Prolines* PAPALLAPAVLLGAALGLGLGLWLGCRAG          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 35, GLY, HA1 A, 32, VAL, O 3.30Å
B, 36, ALA, HA A, 35, GLY, O 2.59Å
B, 39, GLY, HA1 A, 36, ALA, O 2.98Å
A, 35, GLY, HA1 B, 32, VAL, O 3.30Å
A, 36, ALA, HA B, 35, GLY, O 2.59Å
A, 39, GLY, HA1 B, 36, ALA, O 2.98Å