Thyroxine 5-deiodinase

UniProt data

UniProt name: IOD3_HUMAN (P55073)

Gene name: DIO3; Synonyms=ITDI3, TXDI3

Organism: Homo sapiens (Human).

Function: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. {ECO:0000269|PubMed:7593630}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:12419801}; Single-pass type II membrane protein {ECO:0000269|PubMed:12419801}. Endosome membrane {ECO:0000269|PubMed:12419801}; Single-pass type II membrane protein {ECO:0000269|PubMed:12419801}.

Transmembrane domains:

  1. 45-67, 23 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 304 AA

10 20 30 40 50 60 MPRQATSRLV VGEGEGSQGA SGPAATMLRS LLLHSLRLCA QTASCLVLFP RFLGTAFMLW 70 80 90 100 110 120 LLDFLCIRKH FLGRRRRGQP EPEVELNSEG EEVPPDDPPI CVSDDNRLCT LASLKAVWHG 130 140 150 160 170 180 QKLDFFKQAH EGGPAPNSEV VLPDGFQSQH ILDYAQGNRP LVLNFGSCTU PPFMARMSAF 190 200 210 220 230 240 QRLVTKYQRD VDFLIIYIEE AHPSDGWVTT DSPYIIPQHR SLEDRVSAAR VLQQGAPGCA 250 260 270 280 290 300 LVLDTMANSS SSAYGAYFER LYVIQSGTIM YQGGRGPDGY QVSELRTWLE RYDEQLHGAR PRRV

CATM prediction

The sequence of IOD3_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
          50        60        7
   +    |    +    |    +    |		 (Å) (°) (Å) (°)  
Model 1 TASCLVLFARFLGTAFMLWLLDFLCIRKH 6.8 -50.0° 2.9 46.9 -24.6
Prolines* TASCLVLFPRFLGTAFMLWLLDFLCIRKH          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 51, ARG, HA A, 50, ALA, O 2.87Å
B, 54, GLY, HA1 A, 51, ARG, O 2.87Å
A, 51, ARG, HA B, 50, ALA, O 2.87Å
A, 54, GLY, HA1 B, 51, ARG, O 2.87Å