Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2

UniProt data

UniProt name: NDST2_HUMAN (P52849)

Gene name: NDST2; Synonyms=HSST2

Organism: Homo sapiens (Human).

Function: Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000269|PubMed:10758005, ECO:0000269|PubMed:12634318, ECO:0000269|PubMed:16343444, ECO:0000269|PubMed:22660413}.

Subcellular location: Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.

Transmembrane domains:

  1. 19-39, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 883 AA

10 20 30 40 50 60 MLQLWKVVRP ARQLELHRLI LLLIAFSLGS MGFLAYYVST SPKAKEPLPL PLGDCSSGGA 70 80 90 100 110 120 AGPGPARPPV PPRPPRPPET ARTEPVVLVF VESAYSQLGQ EIVAILESSR FRYSTELAPG 130 140 150 160 170 180 RGDMPTLTDN THGRYVLVIY ENLLKYVNLD AWSRELLDRY CVEYGVGIIG FFRAHEHSLL 190 200 210 220 230 240 SAQLKGFPLF LHSNLGLRDY QVNPSAPLLH LTRPSRLEPG PLPGDDWTIF QSNHSTYEPV 250 260 270 280 290 300 LLASLRPAEP AVPGPVLRRA RLPTVVQDLG LHDGIQRVLF GHGLSFWLHK LIFVDAVAYL 310 320 330 340 350 360 TGKRLCLDLD RYILVDIDDI FVGKEGTRMK VADVEALLTT QNKLRTLVPN FTFNLGFSGK 370 380 390 400 410 420 FYHTGTEEED AGDDMLLKHR KEFWWFPHMW SHMQPHLFHN RSVLADQMRL NKQFALEHGI 430 440 450 460 470 480 PTDLGYAVAP HHSGVYPIHT QLYEAWKSVW GIQVTSTEEY PHLRPARYRR GFIHNGIMVL 490 500 510 520 530 540 PRQTCGLFTH TIFYNEYPGG SRELDRSIRG GELFLTVLLN PISIFMTHLS NYGNDRLGLY 550 560 570 580 590 600 TFESLVRFLQ CWTRLRLQTL PPVPLAQKYF ELFPQERSPL WQNPCDDKRH KDIWSKEKTC 610 620 630 640 650 660 DRLPKFLIVG PQKTGTTAIH FFLSLHPAVT SSFPSPSTFE EIQFFNSPNY HKGIDWYMDF 670 680 690 700 710 720 FPVPSNASTD FLFEKSATYF DSEVVPRRGA ALLPRAKIIT VLTNPADRAY SWYQHQRAHG 730 740 750 760 770 780 DPVALNYTFY QVISASSQTP LALRSLQNRC LVPGYYSTHL QRWLTYYPSG QLLIVDGQEL 790 800 810 820 830 840 RTNPAASMES IQKFLGITPF LNYTRTLRFD DDKGFWCQGL EGGKTRCLGR SKGRRYPDMD 850 860 870 880 TESRLFLTDF FRNHNLELSK LLSRLGQPVP SWLREELQHS SLG

CATM prediction

The sequence of NDST2_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
      20        30        40 
    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 LHRLILLLIAFSLGSMGFLAYYVSTSA 6.7 -55.3° 2.4 48.0 -25.8
Prolines* LHRLILLLIAFSLGSMGFLAYYVSTSP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 26, PHE, HA A, 25, ALA, O 2.93Å
B, 29, GLY, HA1 A, 26, PHE, O 2.63Å
A, 26, PHE, HA B, 25, ALA, O 2.93Å
A, 29, GLY, HA1 B, 26, PHE, O 2.63Å
A, 37, TYR, HH B, 37, TYR, OH 2.19Å