E3 ubiquitin-protein ligase RNF144A

UniProt data

UniProt name: R144A_HUMAN (P50876)

Gene name: RNF144A; Synonyms=KIAA0161, RNF144, UBCE7IP4

Organism: Homo sapiens (Human).

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC. {ECO:0000250, ECO:0000269|PubMed:24979766}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:24979766}; Single-pass membrane protein {ECO:0000305}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:24979766}.

Transmembrane domains:

  1. 250-270, 21 AA, Helical. {ECO:0000255}.

Sequence: 292 AA

10 20 30 40 50 60 MTTTRYRPTW DLALDPLVSC KLCLGEYPVE QMTTIAQCQC IFCTLCLKQY VELLIKEGLE 70 80 90 100 110 120 TAISCPDAAC PKQGHLQENE IECMVAAEIM QRYKKLQFER EVLFDPCRTW CPASTCQAVC 130 140 150 160 170 180 QLQDVGLQTP QPVQCKACRM EFCSTCKASW HPGQGCPETM PITFLPGETS AAFKMEEDDA 190 200 210 220 230 240 PIKRCPKCKV YIERDEGCAQ MMCKNCKHAF CWYCLESLDD DFLLIHYDKG PCRNKLGHSR 250 260 270 280 290 ASVIWHRTQV VGIFAGFGLL LLVASPFLLL ATPFVLCCKC KCSKGDDDPL PT

CATM prediction

The sequence of R144A_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
     250       260       270 
   |    +    |    +    |   		 (Å) (°) (Å) (°)  
Model 1 RTQVVGIFAGFGLLLLVASAFLLLATA 7.2 -51.0° 2.4 45.0 -9.9
Model 2 RTQVVGIFAGFGLLLLVASAFLLLATA 7.3 -30.0° 4.7 39.0 -7.7
Prolines* RTQVVGIFAGFGLLLLVASPFLLLATP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session