Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

UniProt data

UniProt name: OST48_HUMAN (P39656)

Gene name: DDOST; Synonyms=KIAA0115, OST48; ORFNames=OK/SW-cl.45

Organism: Homo sapiens (Human).

Function: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Transmembrane domains:

  1. 428-447, 20 AA, Helical. {ECO:0000255}.

Sequence: 456 AA

10 20 30 40 50 60 MGYFRCARAG SFGRRRKMEP STAARAWALF WLLLPLLGAV CASGPRTLVL LDNLNVRETH 70 80 90 100 110 120 SLFFRSLKDR GFELTFKTAD DPSLSLIKYG EFLYDNLIIF SPSVEDFGGN INVETISAFI 130 140 150 160 170 180 DGGGSVLVAA SSDIGDPLRE LGSECGIEFD EEKTAVIDHH NYDISDLGQH TLIVADTENL 190 200 210 220 230 240 LKAPTIVGKS SLNPILFRGV GMVADPDNPL VLDILTGSST SYSFFPDKPI TQYPHAVGKN 250 260 270 280 290 300 TLLIAGLQAR NNARVIFSGS LDFFSDSFFN SAVQKAAPGS QRYSQTGNYE LAVALSRWVF 310 320 330 340 350 360 KEEGVLRVGP VSHHRVGETA PPNAYTVTDL VEYSIVIQQL SNGKWVPFDG DDIQLEFVRI 370 380 390 400 410 420 DPFVRTFLKK KGGKYSVQFK LPDVYGVFQF KVDYNRLGYT HLYSSTQVSV RPLQHTQYER 430 440 450 FIPSAYPYYA SAFSMMLGLF IFSIVFLHMK EKEKSD

CATM prediction

The sequence of OST48_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       430       440       4
+    |    +    |    +    |		 (Å) (°) (Å) (°)  
Model 1 AYAYYASAFSMMLGLFIFSIVFLHMK 7.2 -51.3° 3.3 47.6 -3.7
Prolines* AYPYYASAFSMMLGLFIFSIVFLHMK          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 434, SER, HG1 A, 431, SER, OG 2.01Å
B, 431, SER, HA A, 434, SER, OG 3.06Å
B, 435, MET, HA A, 434, SER, O 3.04Å
B, 438, GLY, HA1 A, 435, MET, O 3.24Å
A, 431, SER, HA B, 434, SER, OG 3.07Å
A, 435, MET, HA B, 434, SER, O 3.04Å
A, 438, GLY, HA1 B, 435, MET, O 3.24Å