Phosphatidylinositol N-acetylglucosaminyltransferase subunit A

UniProt data

UniProt name: PIGA_HUMAN (P37287)

Gene name: PIGA

Organism: Homo sapiens (Human).

Function: Necessary for the synthesis of N-acetylglucosaminyl- phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

Subcellular location: Endoplasmic reticulum membrane; Single-pass membrane protein.

Transmembrane domains:

  1. 422-442, 21 AA, Helical. {ECO:0000255}.

Sequence: 484 AA

10 20 30 40 50 60 MACRGGAGNG HRASATLSRV SPGSLYTCRT RTHNICMVSD FFYPNMGGVE SHIYQLSQCL 70 80 90 100 110 120 IERGHKVIIV THAYGNRKGI RYLTSGLKVY YLPLKVMYNQ STATTLFHSL PLLRYIFVRE 130 140 150 160 170 180 RVTIIHSHSS FSAMAHDALF HAKTMGLQTV FTDHSLFGFA DVSSVLTNKL LTVSLCDTNH 190 200 210 220 230 240 IICVSYTSKE NTVLRAALNP EIVSVIPNAV DPTDFTPDPF RRHDSITIVV VSRLVYRKGI 250 260 270 280 290 300 DLLSGIIPEL CQKYPDLNFI IGGEGPKRII LEEVRERYQL HDRVRLLGAL EHKDVRNVLV 310 320 330 340 350 360 QGHIFLNTSL TEAFCMAIVE AASCGLQVVS TRVGGIPEVL PENLIILCEP SVKSLCEGLE 370 380 390 400 410 420 KAIFQLKSGT LPAPENIHNI VKTFYTWRNV AERTEKVYDR VSVEAVLPMD KRLDRLISHC 430 440 450 460 470 480 GPVTGYIFAL LAVFNFLFLI FLRWMTPDSI IDVAIDATGP RGAWTNNYSH SKRGGENNEI SETR

CATM prediction

The sequence of PIGA_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
   420       430       440   
 |    +    |    +    |    +		 (Å) (°) (Å) (°)  
Model 1 HCGAVTGYIFALLAVFNFLFLIFLRWM 6.5 -40.2° 4.5 43.9 -21.9
Model 2 HCGAVTGYIFALLAVFNFLFLIFLRWM 6.5 -50.7° 6.0 44.8 -8.8
Prolines* HCGPVTGYIFALLAVFNFLFLIFLRWM          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 425, GLY, HA1 A, 422, ALA, O 2.84Å
B, 429, ALA, HA A, 426, TYR, OH 3.37Å
A, 425, GLY, HA1 B, 422, ALA, O 2.84Å
A, 429, ALA, HA B, 426, TYR, OH 3.37Å