Long-chain-fatty-acid--CoA ligase 1

UniProt data

UniProt name: ACSL1_HUMAN (P33121)

Gene name: ACSL1; Synonyms=FACL1, FACL2, LACS, LACS1, LACS2

Organism: Homo sapiens (Human).

Function: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.

Subcellular location: Mitochondrion outer membrane {ECO:0000250}; Single-pass type III membrane protein {ECO:0000250}. Peroxisome membrane {ECO:0000250}; Single-pass type III membrane protein {ECO:0000250}. Microsome membrane {ECO:0000250}; Single-pass type III membrane protein {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type III membrane protein {ECO:0000250}.

Transmembrane domains:

  1. 25-45, 21 AA, Helical; Signal-anchor for type III membrane protein. {ECO:0000255}.

Sequence: 698 AA

10 20 30 40 50 60 MQAHELFRYF RMPELVDFRQ YVRTLPTNTL MGFGAFAALT TFWYATRPKP LKPPCDLSMQ 70 80 90 100 110 120 SVEVAGSGGA RRSALLDSDE PLVYFYDDVT TLYEGFQRGI QVSNNGPCLG SRKPDQPYEW 130 140 150 160 170 180 LSYKQVAELS ECIGSALIQK GFKTAPDQFI GIFAQNRPEW VIIEQGCFAY SMVIVPLYDT 190 200 210 220 230 240 LGNEAITYIV NKAELSLVFV DKPEKAKLLL EGVENKLIPG LKIIVVMDAY GSELVERGQR 250 260 270 280 290 300 CGVEVTSMKA MEDLGRANRR KPKPPAPEDL AVICFTSGTT GNPKGAMVTH RNIVSDCSAF 310 320 330 340 350 360 VKATENTVNP CPDDTLISFL PLAHMFERVV ECVMLCHGAK IGFFQGDIRL LMDDLKVLQP 370 380 390 400 410 420 TVFPVVPRLL NRMFDRIFGQ ANTTLKRWLL DFASKRKEAE LRSGIIRNNS LWDRLIFHKV 430 440 450 460 470 480 QSSLGGRVRL MVTGAAPVSA TVLTFLRAAL GCQFYEGYGQ TECTAGCCLT MPGDWTAGHV 490 500 510 520 530 540 GAPMPCNLIK LVDVEEMNYM AAEGEGEVCV KGPNVFQGYL KDPAKTAEAL DKDGWLHTGD 550 560 570 580 590 600 IGKWLPNGTL KIIDRKKHIF KLAQGEYIAP EKIENIYMRS EPVAQVFVHG ESLQAFLIAI 610 620 630 640 650 660 VVPDVETLCS WAQKRGFEGS FEELCRNKDV KKAILEDMVR LGKDSGLKPF EQVKGITLHP 670 680 690 ELFSIDNGLL TPTMKAKRPE LRNYFRSQID DLYSTIKV

CATM prediction

The sequence of ACSL1_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
          30        40       
   +    |    +    |    +   		 (Å) (°) (Å) (°)  
Model 1 VRTLATNTLMGFGAFAALTTFWYATRA 6.8 -54.9° 0.9 50.1 -18.9
Prolines* VRTLPTNTLMGFGAFAALTTFWYATRP          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 31, MET, HA A, 30, LEU, O 3.44Å
B, 34, GLY, HA1 A, 31, MET, O 2.82Å
B, 35, ALA, HA A, 34, GLY, O 3.18Å
A, 31, MET, HA B, 30, LEU, O 3.44Å
A, 34, GLY, HA1 B, 31, MET, O 2.82Å
A, 35, ALA, HA B, 34, GLY, O 3.18Å