NKG2-D type II integral membrane protein

UniProt data

UniProt name: NKG2D_HUMAN (P26718)

Gene name: KLRK1; Synonyms=D12S2489E, NKG2D

Organism: Homo sapiens (Human).

Function: Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. {ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:11224526, ECO:0000269|PubMed:11777960, ECO:0000269|PubMed:15240696, ECO:0000269|PubMed:21898152, ECO:0000269|PubMed:23298206}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:11015446, ECO:0000269|PubMed:15294961}; Single- pass type II membrane protein {ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:11015446, ECO:0000269|PubMed:15294961}. Note=Colocalized with HCST on the cell surface.

Transmembrane domains:

  1. 52-72, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 216 AA

10 20 30 40 50 60 MGWIRGRRSR HSWEMSEFHN YNLDLKKSDF STRWQKQRCP VVKSKCRENA SPFFFCCFIA 70 80 90 100 110 120 VAMGIRFIIM VAIWSAVFLN SLFNQEVQIP LTESYCGPCP KNWICYKNNC YQFFDESKNW 130 140 150 160 170 180 YESQASCMSQ NASLLKVYSK EDQDLLKLVK SYHWMGLVHI PTNGSWQWED GSILSPNLLT 190 200 210 IIEMQKGDCA LYASSFKGYI ENCSTPNTYI CMQRTV

CATM prediction

The sequence of NKG2D_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
   50        60        70    
 |    +    |    +    |    +		 (Å) (°) (Å) (°)  
Model 1 NASAFFFCCFIAVAMGIRFIIMVAIWS 7.4 -54.0° 3.6 49.1 -0.9
Prolines* NASPFFFCCFIAVAMGIRFIIMVAIWS          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 61, VAL, HA A, 60, ALA, O 3.36Å
B, 64, GLY, HA1 A, 61, VAL, O 3.48Å
A, 61, VAL, HA B, 60, ALA, O 3.36Å
A, 64, GLY, HA1 B, 61, VAL, O 3.48Å